ArticleBiochemistry2013
Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae.
Article in Biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- A reductive uric acid degradation pathway in anaerobic bacteria.Life metabolism · 2025Article
- Genetic landscape and evolution of Acinetobacter pittii, an underestimated emerging nosocomial pathogen.Communications biology · 2025Article
- Structural and Functional Characterization of a Novel Class A Flavin Monooxygenase fromBiochemistry · 2024Article
- Ligand bound structure of a 6-hydroxynicotinic acid 3-monooxygenase provides mechanistic insights.Archives of biochemistry and biophysics · 2024Article
- Antioxidant activity of novel nitrogen scaffold with docking investigation and correlation of DFT stimulation.RSC advances · 2023Article
- Discovery and biosynthesis of karnamicins as angiotensin converting enzyme inhibitors.Nature communications · 2023Article
- Structural basis of the stereoselective formation of the spirooxindole ring in the biosynthesis of citrinadins.Nature communications · 2021Article
- Structural analyses of the Group A flavin-dependent monooxygenase PieE reveal a sliding FAD cofactor conformation bridging OUT and IN conformations.The Journal of biological chemistry · 2020Article
- Molecular Basis for Spirocycle Formation in the Paraherquamide Biosynthetic Pathway.Journal of the American Chemical Society · 2020Article
- Catalysis and Structure of Zebrafish Urate Oxidase Provide Insights into the Origin of Hyperuricemia in Hominoids.Scientific reports · 2016Article
- The identification of an integral membrane, cytochrome c urate oxidase completes the catalytic repertoire of a therapeutic enzyme.Scientific reports · 2015Article
- Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli.Toxins · 2013Article
- The purinosome, a multi-protein complex involved in the de novo biosynthesis of purines in humans.Chemical communications (Cambridge, England) · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 and 2.2 Å, respectively. We have also determined the structure of the R204Q variant at 2.0 Å resolution in the absence of uric acid. The variant structure is very similar to that of wild-type HpxO except for the conformation of Arg103, which interacts with FAD in the variant but not in the wild-type structure. Interestingly, the R204Q variant results in the uncoupling of nicotinamide adenine dinucleotide oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid, activating it for the oxygen transfer. On the basis of these data, a mechanism for this reaction consisting of a nucleophilic attack of the urate anion on the flavin hydroperoxide resulting in the formation of 5-hydroxyisourate is proposed.
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