ArticleThyroid : official journal of the American Thyroid Association2013
Hepatitis C virus infection of a thyroid cell line: implications for pathogenesis of hepatitis C virus and thyroiditis.
Article in Thyroid : official journal of the American Thyroid Association, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Unveiling the mediating role of immune cells in the link between pathogen infections and Hashimoto's thyroiditis.The Journal of international medical research · 2026Article
- Review
- Clinical and molecular impact of concurrent thyroid autoimmune disease and thyroid cancer: From the bench to bedside.Reviews in endocrine & metabolic disorders · 2024Review
- Viruses and Endocrine Diseases.Microorganisms · 2023Review
- High Prevalence of Common Human Viruses in Thyroid Tissue.Frontiers in endocrinology · 2022Article
- The interplay between thyroid and liver: implications for clinical practice.Journal of endocrinological investigation · 2020Review
- Hepatitis C Virus Infection of Human Thyrocytes: Metabolic, Hormonal, and Immunological Implications.The Journal of clinical endocrinology and metabolism · 2020Article
- Article
- Thyroid Disorders in Homozygous β-Thalassemia: Current Knowledge, Emerging Issues and Open Problems.Mediterranean journal of hematology and infectious diseases · 2019Review
- Immunomodulation of CXCL10 Secretion by Hepatitis C Virus: Could CXCL10 Be a Prognostic Marker of Chronic Hepatitis C?Journal of immunology research · 2019Review
- Thyroid and celiac disease in pediatric age: a literature review.Acta bio-medica : Atenei Parmensis · 2018Review
- Endocrine disorders associated with hepatitis C virus chronic infection.Reviews in endocrine & metabolic disorders · 2018Review
- A preliminary analysis of hepatitis C virus in pancreatic islet cells.Virology journal · 2017Article
- Hepatitis C Virus E2 Protein Induces Upregulation of IL-8 Pathways and Production of Heat Shock Proteins in Human Thyroid Cells.The Journal of clinical endocrinology and metabolism · 2017Article
- Thyroid Autoimmunity: Role of Anti-thyroid Antibodies in Thyroid and Extra-Thyroidal Diseases.Frontiers in immunology · 2017Review
- Thyroid Dysfunction in Non-Interferon Treated Hepatitis C Patients Residing in Hepatitis Endemic Area.BioMed research international · 2017Article
- Thyroid Involvement in Hepatitis C Virus-Infected Patients with/without Mixed Cryoglobulinemia.Frontiers in endocrinology · 2017Review
- Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis.Journal of medical microbiology · 2016Article
- Hepatitis C virus infection and thyroid autoimmune disorders: A model of interactions between the host and the environment.World journal of hepatology · 2016Review
- Systemic manifestations of hepatitis C infection.Infectious agents and cancer · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAutoimmune and non-autoimmune thyroiditis frequently occur in persons with hepatitis C virus (HCV) infection. Treatment with interferon alpha (IFNα) is also associated with significant risk for the development of thyroiditis. To explore HCV-thyroid interactions at a cellular level, we evaluated whether a human thyroid cell line (ML1) could be infected productively with HCV in vitro. METHODS AND
resultsML1 cells showed robust surface expression of the major HCV receptor CD81. Using a highly sensitive, strand-specific reverse transcription polymerase chain reaction assay, positive-sense and negative-sense HCV RNA were detected in ML1 cell lysates at days 3, 7, and 14 postinfection with HCV. HCV core protein was expressed at high levels in ML1 supernatants at days 1, 3, 5, 7, and 14 postinfection. The nonstructural protein NS5A was also detected in ML1 cell lysates by Western blotting. HCV entry into ML1 cells was shown to be dependent on the HCV entry factors CD81 and SR-B1/CLA1, while IFNα inhibited HCV replication in ML1 cells in a dose-dependent manner. Supernatants from HCV-infected ML1 cells were able to infect fresh ML1 cells productively, suggesting that infectious virions could be transferred from infected to naïve thyroid cells in vivo. Additionally, HCV infection of ML1 cells led to increased expression of the pro-inflammatory cytokine IL-8.
conclusionsFor the first time, we have demonstrated that HCV can infect human thyroid cells in vitro. These findings strongly suggest that HCV infection of thyrocytes may play a role in the association between chronic HCV infection and thyroid autoimmunity. Furthermore, the thyroid may serve as an extrahepatic reservoir for HCV viral replication, thus contributing to the persistence of viral infection and to the development of thyroid autoimmunity.
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