ArticleMalaria journal2012
Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Antibody correlates of risk of clinical malaria in an area of low and unstable malaria transmission in western Kenya.Malaria journal · 2025Article
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- Serological Markers of Exposure to Plasmodium falciparum and Plasmodium vivax Infection in Southwestern Ethiopia.The American journal of tropical medicine and hygiene · 2023Article
- Diagnostic applications of microsphere-based flow cytometry: A review.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Antibody Correlates of Protection from ClinicalThe American journal of tropical medicine and hygiene · 2020Review
- Antibody Profiles toThe American journal of tropical medicine and hygiene · 2020Article
- Measuring antibody avidity to Plasmodium falciparum merozoite antigens using a multiplex immunoassay approach.Malaria journal · 2020Article
- Quality control of multiplex antibody detection in samples from large-scale surveys: the example of malaria in Haiti.Scientific reports · 2020Article
- Autoantibody levels are associated with acute kidney injury, anemia and post-discharge morbidity and mortality in Ugandan children with severe malaria.Scientific reports · 2019Article
- Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure.BMC medicine · 2019Article
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- Optimisation and standardisation of a multiplex immunoassay of diverseWellcome open research · 2019Article
- Effect of TTC Treatment on Immunohistochemical Quantification of Collagen IV in Rat Brains after Stroke.Translational stroke research · 2018Article
- Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens.Journal of immunological methods · 2018Article
- Decrease in Numbers of Naive and Resting B Cells in HIV-Infected Kenyan Adults Leads to a Proportional Increase in Total andJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Serological markers to measure recent changes in malaria at population level in Cambodia.Malaria journal · 2016Article
- Multi-epitope chimeric antigen used as a serological marker to estimate Plasmodium falciparum transmission intensity in the border area of China-Myanmar.Infectious diseases of poverty · 2016Article
- The Statistical Value of Raw Fluorescence Signal in Luminex xMAP Based Multiplex Immunoassays.Scientific reports · 2016Article
- Multiple comparisons analysis of serological data from an area of low Plasmodium falciparum transmission.Malaria journal · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundMultiplex cytometric bead assay (CBA) have a number of advantages over ELISA for antibody testing, but little information is available on standardization and validation of antibody CBA to multiple Plasmodium falciparum antigens. The present study was set to determine optimal parameters for multiplex testing of antibodies to P. falciparum antigens, and to compare results of multiplex CBA to ELISA.
methodsAntibodies to ten recombinant P. falciparum antigens were measured by CBA and ELISA in samples from 30 individuals from a malaria endemic area of Kenya and compared to known positive and negative control plasma samples. Optimal antigen amounts, monoplex vs multiplex testing, plasma dilution, optimal buffer, number of beads required were assessed for CBA testing, and results from CBA vs. ELISA testing were compared.
resultsOptimal amounts for CBA antibody testing differed according to antigen. Results for monoplex CBA testing correlated strongly with multiplex testing for all antigens (r = 0.88-0.99, P values from <0.0001 - 0.004), and antibodies to variants of the same antigen were accurately distinguished within a multiplex reaction. Plasma dilutions of 1:100 or 1:200 were optimal for all antigens for CBA testing. Plasma diluted in a buffer containing 0.05% sodium azide, 0.5% polyvinylalcohol, and 0.8% polyvinylpyrrolidone had the lowest background activity. CBA median fluorescence intensity (MFI) values with 1,000 antigen-conjugated beads/well did not differ significantly from MFI with 5,000 beads/well. CBA and ELISA results correlated well for all antigens except apical membrane antigen-1 (AMA-1). CBA testing produced a greater range of values in samples from malaria endemic areas and less background reactivity for blank samples than ELISA.
conclusionWith optimization, CBA may be the preferred method of testing for antibodies to P. falciparum antigens, as CBA can test for antibodies to multiple recombinant antigens from a single plasma sample and produces a greater range of values in positive samples and lower background readings for blank samples than ELISA.
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