ArticleGenome biology2012
A microRNA network regulates proliferative timing and extracellular matrix synthesis during cellular quiescence in fibroblasts.
Article in Genome biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 70 citations in OpenAlex.
- Starvation activates ECM-remodeling gene transcription and putative enhancers in fibroblasts despite inducing quiescence.Cell reports · 2025Article
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- Nutrient starvation activates ECM remodeling gene enhancers associated with inflammatory bowel disease risk in fibroblasts.bioRxiv : the preprint server for biology · 2024Article
- Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In Vitro.International journal of molecular sciences · 2023Article
- Chronic wounds.Nature reviews. Disease primers · 2022Review
- Biochemical composition of the glomerular extracellular matrix in patients with diabetic kidney disease.World journal of diabetes · 2022Review
- Histone N-terminal acetyltransferase NAA40 links one-carbon metabolism to chemoresistance.Oncogene · 2022Article
- Chromatin Dynamics During Entry to Quiescence and Compromised Functionality in Cancer Cells.Results and problems in cell differentiation · 2022Article
- Review
- Review
- Cancer-secreted miRNAs regulate amino-acid-induced mTORC1 signaling and fibroblast protein synthesis.EMBO reports · 2021Article
- Is There a Histone Code for Cellular Quiescence?Frontiers in cell and developmental biology · 2021Review
- A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth.Journal of visualized experiments : JoVE · 2020Article
- Cellular Mechanisms and Regulation of Quiescence.Developmental cell · 2020Review
- Repression of Irs2 by let-7 miRNAs is essential for homeostasis of the telencephalic neuroepithelium.The EMBO journal · 2020Article
- Antagonism between splicing and microprocessor complex dictates the serum-induced processing of lnc-RNA (New York, N.Y.) · 2020Article
- Let-7 derived from endometrial extracellular vesicles is an important inducer of embryonic diapause in mice.Science advances · 2020Article
- Cell senescence altered the miRNA expression profile in porcine angular aqueous plexus cells.Molecular vision · 2020Article
- Regulation of Cell Cycle Entry and Exit: A Single Cell Perspective.Comprehensive Physiology · 2019Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough quiescence (reversible cell cycle arrest) is a key part in the life history and fate of many mammalian cell types, the mechanisms of gene regulation in quiescent cells are poorly understood. We sought to clarify the role of microRNAs as regulators of the cellular functions of quiescent human fibroblasts.
resultsUsing microarrays, we discovered that the expression of the majority of profiled microRNAs differed between proliferating and quiescent fibroblasts. Fibroblasts induced into quiescence by contact inhibition or serum starvation had similar microRNA profiles, indicating common changes induced by distinct quiescence signals. By analyzing the gene expression patterns of microRNA target genes with quiescence, we discovered a strong regulatory function for miR-29, which is downregulated with quiescence. Using microarrays and immunoblotting, we confirmed that miR-29 targets genes encoding collagen and other extracellular matrix proteins and that those target genes are induced in quiescence. In addition, overexpression of miR-29 resulted in more rapid cell cycle re-entry from quiescence. We also found that let-7 and miR-125 were upregulated in quiescent cells. Overexpression of either one alone resulted in slower cell cycle re-entry from quiescence, while the combination of both together slowed cell cycle re-entry even further.
conclusionsmicroRNAs regulate key aspects of fibroblast quiescence including the proliferative state of the cells as well as their gene expression profiles, in particular, the induction of extracellular matrix proteins in quiescent fibroblasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.