ArticleBiotechnology for biofuels2012
RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803.
Article in Biotechnology for biofuels, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 80 citations in OpenAlex.
- The regulatory impact of serine/threonine-specific protein phosphorylation among cyanobacteria.Frontiers in plant science · 2025Review
- Unravelling the transcriptome response of Enterobacter sp. S-33 under varying temperature.Archives of microbiology · 2024Article
- Expression of tardigrade disordered proteins impacts the tolerance to biofuels in a model cyanobacteriumFrontiers in microbiology · 2022Article
- Recent advances in synthetic biology of cyanobacteria for improved chemicals production.Bioengineered · 2020Review
- Impact of RNase E and RNase J on Global mRNA Metabolism in the CyanobacteriumFrontiers in microbiology · 2020Article
- RNA-Seq-Based Comparative Transcriptome Analysis Highlights New Features of the Heat-Stress Response in the Extremophilic BacteriumInternational journal of molecular sciences · 2019Article
- Genome Features and Biochemical Characteristics of a Robust, Fast Growing and Naturally Transformable Cyanobacterium Synechococcus elongatus PCC 11801 Isolated from India.Scientific reports · 2018Article
- Evaluation of the ethanol tolerance for wild and mutantBiotechnology reports (Amsterdam, Netherlands) · 2018Article
- Regulation Mechanism Mediated byFrontiers in microbiology · 2018Article
- Enabling the democratization of the genomics revolution with a fully integrated web-based bioinformatics platform.Nucleic acids research · 2017Article
- Metabolic responses to ethanol and butanol inBiotechnology for biofuels · 2017Article
- Systematic and functional identification of small non-coding RNAs associated with exogenous biofuel stress in cyanobacteriumBiotechnology for biofuels · 2017Article
- Functional Diversity of Transcriptional Regulators in the CyanobacteriumFrontiers in microbiology · 2017Article
- A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance ofBiotechnology for biofuels · 2017Article
- Cyanobacteria as Chassis for Industrial Biotechnology: Progress and Prospects.Life (Basel, Switzerland) · 2016Review
- Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacteriumBiotechnology for biofuels · 2016Article
- Engineering cyanobacteria as photosynthetic feedstock factories.Photosynthesis research · 2015Review
- Photoheterotrophic fluxome in Synechocystis sp. strain PCC 6803 and its implications for cyanobacterial bioenergetics.Journal of bacteriology · 2015Article
- Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories.Biotechnology for biofuels · 2015Article
- Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803.Biotechnology for biofuels · 2015Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
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backgroundFermentation production of biofuel ethanol consumes agricultural crops, which will compete directly with the food supply. As an alternative, photosynthetic cyanobacteria have been proposed as microbial factories to produce ethanol directly from solar energy and CO2. However, the ethanol productivity from photoautotrophic cyanobacteria is still very low, mostly due to the low tolerance of cyanobacterial systems to ethanol stress.
resultsTo build a foundation necessary to engineer robust ethanol-producing cyanobacterial hosts, in this study we applied a quantitative transcriptomics approach with a next-generation sequencing technology, combined with quantitative reverse-transcript PCR (RT-PCR) analysis, to reveal the global metabolic responses to ethanol in model cyanobacterial Synechocystis sp. PCC 6803. The results showed that ethanol exposure induced genes involved in common stress responses, transporting and cell envelope modification. In addition, the cells can also utilize enhanced polyhydroxyalkanoates (PHA) accumulation and glyoxalase detoxication pathway as means against ethanol stress. The up-regulation of photosynthesis by ethanol was also further confirmed at transcriptional level. Finally, we used gene knockout strains to validate the potential target genes related to ethanol tolerance.
conclusionRNA-Seq based global transcriptomic analysis provided a comprehensive view of cellular response to ethanol exposure. The analysis provided a list of gene targets for engineering ethanol tolerance in cyanobacterium Synechocystis.
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Registered trials
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