ArticleBiology open2012
Complex regulation controls Neurogenin3 proteolysis.
Article in Biology open, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Arginine 65 methylation of Neurogenin 3 by PRMT1 is required for pancreatic endocrine development of hESCs.Experimental & molecular medicine · 2023Article
- USP7 controls NGN3 stability and pancreatic endocrine lineage development.Nature communications · 2023Article
- Thioester and Oxyester Linkages in the Ubiquitin System.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Homodimeric and Heterodimeric Interactions among Vertebrate Basic Helix-Loop-Helix Transcription Factors.International journal of molecular sciences · 2021Review
- Cellular functions and molecular mechanisms of non-lysine ubiquitination.Open biology · 2019Review
- Secretagogin protects Pdx1 from proteasomal degradation to control a transcriptional program required for β cell specification.Molecular metabolism · 2018Article
- Article
- Single-Cell Gene Expression Analysis of a Human ESC Model of Pancreatic Endocrine Development Reveals Different Paths to β-Cell Differentiation.Stem cell reports · 2017Article
- Multi-site Neurogenin3 Phosphorylation Controls Pancreatic Endocrine Differentiation.Developmental cell · 2017Article
- Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors.Developmental cell · 2017Article
- Precommitment low-level Neurog3 expression defines a long-lived mitotic endocrine-biased progenitor pool that drives production of endocrine-committed cells.Genes & development · 2016Article
- Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro.The EMBO journal · 2015Article
- Dimerization-driven degradation of C. elegans and human E proteins.Genes & development · 2015Article
- Cell cycle-dependent differentiation dynamics balances growth and endocrine differentiation in the pancreas.PLoS biology · 2015Article
- Cell cycle regulation of proliferation versus differentiation in the central nervous system.Cell and tissue research · 2015Review
- Multi-site phospho-regulation of proneural transcription factors controls proliferation versus differentiation in development and reprogramming.Neurogenesis (Austin, Tex.) · 2015Article
- Lineage selection and plasticity in the intestinal crypt.Current opinion in cell biology · 2014Review
- Complex domain interactions regulate stability and activity of closely related proneural transcription factors.Biochemical and biophysical research communications · 2014Article
- Loss of Fbw7 reprograms adult pancreatic ductal cells into α, δ, and β cells.Cell stem cell · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ubiquitin proteasome system (UPS) is known to be responsible for the rapid turnover of many transcription factors, where half-life is held to be critical for regulation of transcriptional activity. However, the stability of key transcriptional regulators of development is often very poorly characterised. Neurogenin 3 (Ngn3) is a basic helix-loop-helix transcription factor that plays a central role in specification and differentiation of endocrine cells of the pancreas and gut, as well as spermatogonia and regions of the brain. Here we demonstrate that Ngn3 protein stability is regulated by the ubiquitin proteasome system and that Ngn3 can be ubiquitylated on lysines, the N-terminus and, highly unusually, on non-canonical residues including cysteines and serines/threonines. Rapid turnover of Ngn3 is regulated both by binding to its heterodimeric partner E protein and by the presence of cdk inhibitors. We show that protein half-life does appear to regulate the activity of Ngn3 in vivo, but, unlike the related transcription factor c-myc, ubiquitylation on canonical sites is not a requirement for transcriptional activity of Ngn3. Hence, we characterise an important new level of Ngn3 post-translational control, which may regulate its transcriptional activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.