ArticleEvidence-based complementary and alternative medicine : eCAM2012
Effects of the yangjing capsule extract on steroidogenesis and apoptosis in mouse leydig cells.
Article in Evidence-based complementary and alternative medicine : eCAM, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Seminal vesicles - an overlooked pair of accessory glands in male sexual dysfunction: a narrative review.International journal of impotence research · 2025Review
- Progesterone Induces Apoptosis and Steroidogenesis in Porcine Placental Trophoblasts.Animals : an open access journal from MDPI · 2022Article
- Current Situation and Reconsideration on the Study of Integrated Chinese and Western Medicine Andrology.Chinese journal of integrative medicine · 2020Article
- Discovery of methylpyrimidine ring-fused diterpenoid analogs as a novel testosterone synthesis promoter.RSC advances · 2019Article
- Calretinin Participates in Regulating Steroidogenesis by PLC-CaScientific reports · 2018Article
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- Protective effect of calretinin on testicular Leydig cells via the inhibition of apoptosis.Aging · 2017Article
- Yangjing Capsule Ameliorates Spermatogenesis in Male Mice Exposed to Cyclophosphamide.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- The Stimulative Effect of Yangjing Capsule on Testosterone Synthesis through Nur77 Pathway in Leydig Cells.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- Yangjing Capsule Extract Promotes Proliferation of GC-1 Spg Cells.Evidence-based complementary and alternative medicine : eCAM · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objectives. This study aimed to explore the effect and mechanism of Yangjing capsule on testosterone secretion in mouse Leydig tumor cells (MLTC-1). Methods. MLTC-1 cells were treated with the Yangjing capsule extract for 24 h. The testosterone level in medium was measured by radioimmunoassay. The expression of steroidogenic enzymes (StAR, CYP11A1, and HSD3B) in the cells was examined using real-time RT-PCR and immunoblotting. Additionally, MLTC-1 cells were treated for 48 h in a serum-free medium. The cell viability was measured by MTT assay. The cell cycle and apoptosis were analyzed using flow cytometry. The expression of activated caspase-3 was analyzed using RT-PCR and a colorimetric protease assay. Results. The Yangjing capsule extract increased testosterone production and the expression of StAR, CYP11A1, and HSD3B mRNAs and proteins compared with the control. H89 significantly inhibited these effects. The medicine improved the viability of MLTC-1 cells, decreased the number of cells in G0/G1 phase, and increased the number of cells in S-phase, as well as prevented cell apoptosis by inhibiting caspase-3. Conclusion. The Yangjing capsule can stimulate MLTC-1 cells to secrete testosterone and may be an alternative treatment for diseases characterized by insufficient testosterone production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.