ArticleThe Journal of biological chemistry2013
The RNA polymerase of marine cyanophage Syn5.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Efficient production of a self-amplifying RNA vaccine sequence with the single subunit RNA polymerase fromRNA (New York, N.Y.) · 2026Article
- DragonRNA: Generality of DNA-primed RNA-extension activities by DNA-directed RNA polymerases.Nucleic acids research · 2025Article
- Decoding and reengineering the promoter specificity of T7-like RNA polymerases based on phage genome sequences.Nucleic acids research · 2025Article
- Molecular Biology Applications of Psychrophilic Enzymes: Adaptations, Advantages, Expression, and Prospective.Applied biochemistry and biotechnology · 2024Review
- DNA-terminus-dependent transcription by T7 RNA polymerase and its C-helix mutants.Nucleic acids research · 2024Article
- Expanding the RNA polymerase biocatalyst solution space for mRNA manufacture.Biotechnology journal · 2024Article
- Understanding the impact ofFrontiers in molecular biosciences · 2024Review
- Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics.Frontiers in molecular biosciences · 2024Review
- Genomic Analysis of a New Freshwater Cyanophage Lbo240-yong1 Suggests a New Taxonomic Family of Bacteriophages.Viruses · 2023Article
- Total RNA Synthesis and its Covalent Labeling Innovation.Topics in current chemistry (Cham) · 2022Review
- Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts inRNA biology · 2022Article
- A single mutation attenuates both the transcription termination and RNA-dependent RNA polymerase activity of T7 RNA polymerase.RNA biology · 2021Article
- Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro.Scientific reports · 2019Article
- Article
- Synthesis of 2'-Fluoro RNA by Syn5 RNA polymerase.Nucleic acids research · 2015Article
- The thumb subdomain of yeast mitochondrial RNA polymerase is involved in processivity, transcript fidelity and mitochondrial transcription factor binding.RNA biology · 2015Article
- Syn5 RNA polymerase synthesizes precise run-off RNA products.Nucleic acids research · 2014Article
- Engineered transcriptional systems for cyanobacterial biotechnology.Frontiers in bioengineering and biotechnology · 2014Review
- Bacteriophage T7 DNA polymerase - sequenase.Frontiers in microbiology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
A single subunit DNA-dependent RNA polymerase was identified and purified to apparent homogeneity from cyanophage Syn5 that infects the marine cyanobacteria Synechococcus. Syn5 is homologous to bacteriophage T7 that infects Escherichia coli. Using the purified enzyme its promoter has been identified by examining transcription of segments of Syn5 DNA and sequencing the 5'-termini of the transcripts. Only two Syn5 RNAP promoters, having the sequence 5'-ATTGGGCACCCGTAA-3', are found within the Syn5 genome. One promoter is located within the Syn5 RNA polymerase gene and the other is located close to the right genetic end of the genome. The purified enzyme and its promoter have enabled a determination of the requirements for transcription. Unlike the salt-sensitive bacteriophage T7 RNA polymerase, this marine RNA polymerase requires 160 mm potassium for maximal activity. The optimal temperature for Syn5 RNA polymerase is 24 °C, much lower than that for T7 RNA polymerase. Magnesium is required as a cofactor although some activity is observed with ferrous ions. Syn5 RNA polymerase is more efficient in utilizing low concentrations of ribonucleotides than T7 RNA polymerase.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.