ArticleThe Journal of biological chemistry2013
Pyruvate:ferredoxin oxidoreductase is coupled to light-independent hydrogen production in Chlamydomonas reinhardtii.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
- New functions of pirin proteins and a 2-ketoglutarate: Ferredoxin oxidoreductase ortholog in Bacteroides fragilis metabolism and their impact on antimicrobial susceptibility to metronidazole and amixicile.MicrobiologyOpen · 2024Article
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- Article
- Changing the tracks: screening for electron transfer proteins to support hydrogen production.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Article
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Occurrence, Evolution and Specificities of Iron-Sulfur Proteins and Maturation Factors in Chloroplasts from Algae.International journal of molecular sciences · 2021Review
- Water oxidation by photosystem II is the primary source of electrons for sustained HProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Electron Transfer in Nitrogenase.Chemical reviews · 2020Review
- Photosynthetic Metabolism and Nitrogen Reshuffling Are Regulated by Reversible Cysteine Thiol Oxidation Following Nitrogen Deprivation in Chlamydomonas.Plants (Basel, Switzerland) · 2020Article
- Review
- Designing a modified clostridial 2[4Fe-4S] ferredoxin as a redox coupler to directly link photosystem I with a Pt nanoparticle.Photosynthesis research · 2020Article
- Elimination of the flavodiiron electron sink facilitates long-term HBiotechnology for biofuels · 2019Article
- Flavodiiron-Mediated OPlant physiology · 2018Article
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Influence of the [4Fe-4S] cluster coordinating cysteines on active site maturation and catalytic properties ofChemical science · 2017Article
- Association of Ferredoxin:NADPPhotosynthesis research · 2017Article
- Concerted Up-regulation of Aldehyde/Alcohol Dehydrogenase (ADHE) and Starch inThe Journal of biological chemistry · 2017Article
- On the pathways feeding the HBiotechnology for biofuels · 2017Article
- Crystal structure and biochemical characterization of Chlamydomonas FDX2 reveal two residues that, when mutated, partially confer FDX2 the redox potential and catalytic properties of FDX1.Photosynthesis research · 2016Article
- Identification of the Elusive Pyruvate Reductase of Chlamydomonas reinhardtii Chloroplasts.Plant & cell physiology · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In anaerobiosis, the green alga Chlamydomonas reinhardtii evolves molecular hydrogen (H(2)) as one of several fermentation products. H(2) is generated mostly by the [Fe-Fe]-hydrogenase HYDA1, which uses plant type ferredoxin PETF/FDX1 (PETF) as an electron donor. Dark fermentation of the alga is mainly of the mixed acid type, because formate, ethanol, and acetate are generated by a pyruvate:formate lyase pathway similar to Escherichia coli. However, C. reinhardtii also possesses the pyruvate:ferredoxin oxidoreductase PFR1, which, like pyruvate:formate lyase and HYDA1, is localized in the chloroplast. PFR1 has long been suggested to be responsible for the low but significant H(2) accumulation in the dark because the catalytic mechanism of pyruvate:ferredoxin oxidoreductase involves the reduction of ferredoxin. With the aim of proving the biochemical feasibility of the postulated reaction, we have heterologously expressed the PFR1 gene in E. coli. Purified recombinant PFR1 is able to transfer electrons from pyruvate to HYDA1, using the ferredoxins PETF and FDX2 as electron carriers. The high reactivity of PFR1 toward oxaloacetate indicates that in vivo, fermentation might also be coupled to an anaerobically active glyoxylate cycle. Our results suggest that C. reinhardtii employs a clostridial type H(2) production pathway in the dark, especially because C. reinhardtii PFR1 was also able to allow H(2) evolution in reaction mixtures containing Clostridium acetobutylicum 2[4Fe-4S]-ferredoxin and [Fe-Fe]-hydrogenase HYDA.
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