ArticleDalton transactions (Cambridge, England : 2003)2013
Iron binding activity is essential for the function of IscA in iron-sulphur cluster biogenesis.
Article in Dalton transactions (Cambridge, England : 2003), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Fifty shades of iron: Unorthodox mechanisms of iron acquisition and utilization in blood-stage Plasmodium parasites.PLoS pathogens · 2026Review
- Iron-sulfur cluster biogenesis and regulation of intracellular iron homeostasis in Escherichia coli.Metallomics : integrated biometal science · 2025Review
- Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression.Synthetic and systems biotechnology · 2024Article
- On the evolutionary trail of MagRs.Zoological research · 2024Article
- Unexpected divergence in magnetoreceptor MagR from robin and pigeon linked to two sequence variations.Zoological research · 2024Article
- Towards magnetism in pigeon MagR: Iron- and iron-sulfur binding work indispensably and synergistically.Zoological research · 2023Article
- The rational design of iron-sulfur cluster binding site for prolonged stability in magnetoreceptor MagR.Frontiers in molecular biosciences · 2022Article
- Review
- Identification of an Intermediate Form of Ferredoxin That Binds Only Iron Suggests That Conversion to Holo-Ferredoxin Is Independent of the ISC System in Escherichia coli.Applied and environmental microbiology · 2021Article
- Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis inThe Journal of biological chemistry · 2020Article
- Fe-S cluster biogenesis by the bacterial Suf pathway.Biochimica et biophysica acta. Molecular cell research · 2020Review
- Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria.Frontiers in microbiology · 2020Review
- Zinc Toxicity and Iron-Sulfur Cluster Biogenesis inApplied and environmental microbiology · 2019Article
- Anaerobic Copper Toxicity and Iron-Sulfur Cluster Biogenesis in Escherichia coli.Applied and environmental microbiology · 2017Article
- Physiological roles of bacillithiol in intracellular metal processing.Current genetics · 2016Review
- Bacillithiol has a role in Fe-S cluster biogenesis in Staphylococcus aureus.Molecular microbiology · 2015Article
- Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli.The Journal of biological chemistry · 2015Article
- Copper binding in IscA inhibits iron-sulphur cluster assembly in Escherichia coli.Molecular microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Iron-sulphur cluster biogenesis requires coordinated delivery of iron and sulphur to scaffold proteins, followed by transfer of the assembled clusters from scaffold proteins to target proteins. This complex process is accomplished by a group of dedicated iron-sulphur cluster assembly proteins that are conserved from bacteria to humans. While sulphur in iron-sulphur clusters is provided by L-cysteine via cysteine desulfurase, the iron donor(s) for iron-sulphur cluster assembly remains largely elusive. Here we report that among the primary iron-sulphur cluster assembly proteins, IscA has a unique and strong binding activity for mononuclear iron in vitro and in vivo. Furthermore, the ferric iron centre tightly bound in IscA can be readily extruded by l-cysteine, followed by reduction to ferrous iron for iron-sulphur cluster biogenesis. Substitution of the highly conserved residue tyrosine 40 with phenylalanine (Y40F) in IscA results in a mutant protein that has a diminished iron binding affinity but retains the iron-sulphur cluster binding activity. Genetic complementation studies show that the IscA Y40F mutant is inactive in vivo, suggesting that the iron binding activity is essential for the function of IscA in iron-sulphur cluster biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.