ArticleJournal of the American Chemical Society2013
DNA ligase-mediated translation of DNA into densely functionalized nucleic acid polymers.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- Article
- From liquid-phase synthesis to chemical ligation: preparation of oligonucleotides and their backbone analogs in solution.Nucleic acids research · 2025Review
- Nucleic acid joining enzymes: biological functions and synthetic applications beyond DNA.The Biochemical journal · 2025Review
- Heat-activated growth of metastable and length-defined DNA fibers expands traditional polymer assembly.Nature communications · 2024Article
- Ligase-mediated synthesis of CuChemical science · 2024Article
- Aptamers in neuro-oncology: An emerging therapeutic modality.Neuro-oncology · 2024Review
- Article
- Article
- Generating experimentally unrelated target molecule-binding highly functionalized nucleic-acid polymers using machine learning.Nature communications · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Strategies for developing DNA-encoded libraries beyond binding assays.Nature chemistry · 2022Review
- Isolation of Natural DNA Aptamers for Challenging Small-Molecule Targets, Cannabinoids.Analytical chemistry · 2021Article
- Enzymatic synthesis of hypermodified DNA polymers for sequence-specific display of four different hydrophobic groups.Nucleic acids research · 2020Article
- Review
- Side chain determinants of biopolymer function during selection and replication.Nature chemical biology · 2019Article
- Nucleic Acid-Barcoding Technologies: Converting DNA Sequencing into a Broad-Spectrum Molecular Counter.Angewandte Chemie (International ed. in English) · 2019Review
- Regulation of Protein Activity and Cellular Functions Mediated by Molecularly Evolved Nucleic Acids.Angewandte Chemie (International ed. in English) · 2019Article
- Evolution of sequence-defined highly functionalized nucleic acid polymers.Nature chemistry · 2018Article
- Enzymatic Synthesis of Sequence-Defined Synthetic Nucleic Acid Polymers with Diverse Functional Groups.Angewandte Chemie (International ed. in English) · 2016Article
- DNA-Encoded Solid-Phase Synthesis: Encoding Language Design and Complex Oligomer Library Synthesis.ACS combinatorial science · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a method to translate DNA sequences into densely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization of 5'-phosphorylated trinucleotides containing a wide variety of appended functional groups. This polymerization proceeds sequence specifically along a DNA template and can generate polymers of at least 50 building blocks (150 nucleotides) in length with remarkable efficiency. The resulting single-stranded highly modified nucleic acid is a suitable template for primer extension using deep vent (exo-) DNA polymerase, thereby enabling the regeneration of template DNA. We integrated these capabilities to perform iterated cycles of in vitro translation, selection, and template regeneration on libraries of modified nucleic acid polymers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.