ArticleACS chemical biology2013
Novel acid-activated fluorophores reveal a dynamic wave of protons in the intestine of Caenorhabditis elegans.
Article in ACS chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 29 citations in OpenAlex.
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- Rhodamine-Based Cyclic Hydroxamate as Fluorescent pH Probe for Imaging of Lysosomes.International journal of molecular sciences · 2023Article
- Reciprocating intestinal flows enhance glucose uptake in C. elegans.Scientific reports · 2022Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Distinct roles for twoeLife · 2022Article
- An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in theMicromachines · 2021Article
- Xanthene-based Fluorescence Turn-on Probe for Highly Acidic pH Range in Aqueous Solution.Journal of fluorescence · 2021Article
- The C. elegans CHP1 homolog, pbo-1, functions in innate immunity by regulating the pH of the intestinal lumen.PLoS pathogens · 2020Article
- Optically Robust and Biocompatible Mechanosensitive Upconverting Nanoparticles.ACS central science · 2019Article
- Article
- Caenorhabditis elegans exhibit a coupling between the defecation motor program and directed locomotion.Scientific reports · 2015Article
- H-Rubies, a new family of red emitting fluorescent pH sensors for living cells.Chemical science · 2015Article
- Cycloalkyl-aminomethylrhodamines: pH dependent photophysical properties tuned by cycloalkane ring size.Journal of fluorescence · 2015Article
- Aberrant fat metabolism in Caenorhabditis elegans mutants with defects in the defecation motor program.PloS one · 2015Article
- Anilinomethylrhodamines: pH sensitive probes with tunable photophysical properties by substituent effect.The Journal of organic chemistry · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Unlike the digestive systems of vertebrate animals, the lumen of the alimentary canal of Caenorhabditis elegans is unsegmented and weakly acidic (pH ~4.4), with ultradian fluctuations to pH > 6 every 45-50 s. To probe the dynamics of this acidity, we synthesized novel acid-activated fluorophores termed Kansas Reds. These dicationic derivatives of rhodamine B become concentrated in the lumen of the intestine of living C. elegans and exhibit tunable pKa values (2.3-5.4), controlled by the extent of fluorination of an alkylamine substituent, that allow imaging of a range of acidic fluids in vivo. Fluorescence video microscopy of animals freely feeding on these fluorophores revealed that acidity in the C. elegans intestine is discontinuous; the posterior intestine contains a large acidic segment flanked by a smaller region of higher pH at the posterior-most end. Remarkably, during the defecation motor program, this hot spot of acidity rapidly moves from the posterior intestine to the anterior-most intestine where it becomes localized for up to 7 s every 45-50 s. Studies of pH-insensitive and base-activated fluorophores as well as mutant and transgenic animals revealed that this dynamic wave of acidity requires the proton exchanger PBO-4, does not involve substantial movement of fluid, and likely involves the sequential activation of proton transporters on the apical surface of intestinal cells. Lacking a specific organ that sequesters low pH, C. elegans compartmentalizes acidity by producing of a dynamic hot spot of protons that rhythmically migrates from the posterior to anterior intestine.
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