ArticleMolecular and cellular biology1990
A negative element involved in vimentin gene expression.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.Differentiation; research in biological diversity · 2009Article
- Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta.Experimental cell research · 2007Article
- TGFbeta1 regulation of vimentin gene expression during differentiation of the C2C12 skeletal myogenic cell line requires Smads, AP-1 and Sp1 family members.Biochimica et biophysica acta · 2007Article
- Characterization of a Novel DNA Motif in the Tctex1 and TCP10 Gene Complexes and its Prevalence in the Mouse Genome.Advances in biological research · 2007Article
- Two different negative regulatory elements control the transcription of T-cell activation gene 3 in activated mast cells.The Biochemical journal · 1997Article
- Different vimentin expression in two clones derived from a human colocarcinoma cell line (LoVo) showing different sensitivity to doxorubicin.British journal of cancer · 1995Article
- AP-1, ETS, and transcriptional silencers regulate retinoic acid-dependent induction of keratin 18 in embryonic cells.Molecular and cellular biology · 1994Article
- Characterization of a cis-acting regulatory element which silences expression of the class II-A beta gene in epithelium.The Journal of experimental medicine · 1994Article
- Promoter elements and transcriptional control of the chicken tropomyosin gene [corrected].Nucleic acids research · 1994Article
- Multiple silencer elements are involved in regulating the chicken vimentin gene.Molecular and cellular biology · 1994Article
- Negative regulation of transcription in eukaryotes.The Biochemical journal · 1993Review
- Regulation of the mouse desmin gene: transactivated by MyoD, myogenin, MRF4 and Myf5.Nucleic acids research · 1993Article
- Identification of a negative element in the human vimentin promoter: modulation by the human T-cell leukemia virus type I Tax protein.Molecular and cellular biology · 1993Article
- Nerve growth factor-induced derepression of peripherin gene expression is associated with alterations in proteins binding to a negative regulatory element.Molecular and cellular biology · 1992Article
- Identification of a cis-acting DNA antisilencer element which modulates vimentin gene expression.Molecular and cellular biology · 1992Article
- A position-dependent silencer plays a major role in repressing alpha-fetoprotein expression in human hepatoma.Molecular and cellular biology · 1991Article
- Multiple positive and negative elements regulate human brain creatine kinase gene expression.Nucleic acids research · 1991Article
- Activation and suppression of a cryptic promoter in the intron of the human melanoma-associated ME491 antigen gene.Japanese journal of cancer research : Gann · 1991Article
- Adult chicken alpha-globin gene expression in transfected QT6 quail cells: evidence for a negative regulatory element in the alpha D gene region.Nucleic acids research · 1991Article
- Functional characterization of the human hypoxanthine phosphoribosyltransferase gene promoter: evidence for a negative regulatory element.Molecular and cellular biology · 1991Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vimentin is one member of the intermediate filament multigene family which exhibits both tissue- and developmental stage-specific expression. In vivo, vimentin is expressed in cells of mesenchymal origin. Previously, we identified both enhancer and promoter elements in the chicken vimentin gene which regulate gene expression in a positive manner. In this report, we have identified a 40-base-pair region at -568 base pairs between the proximal and distal enhancer elements which represses transcriptional activity. This silencer region can also repress the heterologous herpes simplex virus thymidine kinase promoter, which is comparable to the vimentin promoter. In addition, the element is able to function in a position- and orientation-independent manner, and the amount of repression is increased by multiple copies. Here we show by gel retardation assays and DNase I footprinting that this region binds a protein in nuclear extracts from HeLa cells. Southwestern (DNA-protein) blot analysis indicates this protein is approximately 95 kilodaltons in size. Moreover, protein distribution and activity mimic the expression pattern of vimentin during myogenesis, i.e., protein binding increases as vimentin gene expression decreases. The silencer region shares strong sequence similarity with 5'-flanking sequences found in both the human and hamster vimentin genes and with other characterized silencer elements, including the human immunodeficiency virus long terminal repeat, rat growth hormone, chicken lysozyme, and rat insulin genes. Thus, a negative element appears to bind a 95-kilodalton protein involved in regulating the tissue-specific expression of the chicken vimentin gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.