ArticleMolecular and cellular biology1990
In vitro posttranslational modification of lamin B cloned from a human T-cell line.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 104 citations in OpenAlex.
- Sensing the squeeze: nuclear mechanotransduction in health and disease.Nucleus (Austin, Tex.) · 2024Review
- Inter-proteomic posttranslational modifications of the SARS-CoV-2 and the host proteins ‒ A new frontier.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- The Genomic Health of Human Pluripotent Stem Cells: Genomic Instability and the Consequences on Nuclear Organization.Frontiers in genetics · 2018Review
- Temporal Changes in Nucleus Morphology, Lamin A/C and Histone Methylation During Nanotopography-Induced Neuronal Differentiation of Stem Cells.Frontiers in bioengineering and biotechnology · 2018Article
- Comparing lamin proteins post-translational relative stability using a 2A peptide-based system reveals elevated resistance of progerin to cellular degradation.Nucleus (Austin, Tex.) · 2016Article
- Confocal Analysis of Nuclear Lamina Behavior during Male Meiosis and Spermatogenesis in Drosophila melanogaster.PloS one · 2016Article
- Human airway epithelia express catalytically active NEU3 sialidase.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Lamins at a glance.Journal of cell science · 2012Review
- LMNA variants cause cytoplasmic distribution of nuclear pore proteins in Drosophila and human muscle.Human molecular genetics · 2012Article
- The role of Drosophila Lamin C in muscle function and gene expression.Development (Cambridge, England) · 2010Article
- Determination of the subcellular distribution of mercaptoundecahydro-closo-dodecaborate (BSH) in human glioblastoma multiforme by electron microscopy.Journal of neuro-oncology · 2002Article
- Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells.The Journal of cell biology · 2001Article
- Granzymes A and B directly cleave lamins and disrupt the nuclear lamina during granule-mediated cytolysis.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells.The Journal of cell biology · 2000Article
- Article
- The beta enolase subunit displays three different patterns of microheterogeneity in human striated muscle.Journal of muscle research and cell motility · 1999Article
- Nopp140 functions as a molecular link between the nucleolus and the coiled bodies.The Journal of cell biology · 1998Article
- Lamin proteolysis facilitates nuclear events during apoptosis.The Journal of cell biology · 1996Article
- Autoantibodies to nuclear envelope antigens in chronic fatigue syndrome.The Journal of clinical investigation · 1996Article
- A chromatin binding site in the tail domain of nuclear lamins that interacts with core histones.The Journal of cell biology · 1995Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line MOLT-4, which encodes nuclear lamin B. The identity of the encoded protein as lamin B was established by both biochemical and immunological criteria. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region. Analysis of mRNA in HL60 and MOLT-4 cells, which express only lamin B, or HeLa cells, which express all three major lamins (A, B, and C), together with the comigration of in vitro-translated product with isolated HeLa cell lamin B by two-dimensional gel electrophoresis, suggests that a single lamin B is expressed in mammalian somatic cells. In vitro translation with the cDNA clone revealed an EDTA-sensitive posttranslational modification which resulted in an increase in the apparent molecular weight to that equivalent to the native in vivo-synthesized lamin B protein. This in vitro modification included incorporation of a product of mevalonolactone and required an intact carboxy terminus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.