ArticleMolecular and cellular biology1990
Myosin light-chain 1/3 gene alternative splicing: cis regulation is based upon a hierarchical compatibility between splice sites.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- A novel mechanism of alternative promoter and splicing regulates the epitope generation of tumor antigen CML66-L.Journal of immunology (Baltimore, Md. : 1950) · 2004Trial
- Finding signals that regulate alternative splicing in the post-genomic era.Genome biology · 2002Review
- Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons.Molecular and cellular biology · 1999Article
- Donor site competition is involved in the regulation of alternative splicing of the rat beta-tropomyosin pre-mRNA.RNA (New York, N.Y.) · 1999Article
- The Sex-lethal early splicing pattern uses a default mechanism dependent on the alternative 5' splice sites.Molecular and cellular biology · 1997Article
- Relative exon affinities and suboptimal splice site signals lead to non-equivalence of two cassette exons.Nucleic acids research · 1995Article
- Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.Molecular and cellular biology · 1993Article
- Elements regulating an alternatively spliced exon of the rat fibronectin gene.Molecular and cellular biology · 1993Article
- The exon trapping assay partly discriminates against alternatively spliced exons.Nucleic acids research · 1993Article
- Differential splicing in mouse thymus generates two forms of terminal deoxynucleotidyl transferase.Nucleic acids research · 1993Article
- Processing of chimeric introns in dicot plants: evidence for a close cooperation between 5' and 3' splice sites.Nucleic acids research · 1992Article
- Exon recognition and nucleocytoplasmic partitioning determine AMPD1 alternative transcript production.Molecular and cellular biology · 1991Article
- Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site.Molecular and cellular biology · 1991Article
- Effect of 5' splice site mutations on splicing of the preceding intron.Molecular and cellular biology · 1990Article
- A novel pathway for alternative splicing: identification of an RNA intermediate that generates an alternative 5' splice donor site not present in the primary transcript of AMPD1.Molecular and cellular biology · 1990Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms involved in the selective joining of appropriate 5' and 3' splice sites are still poorly understood in both constitutive and alternatively spliced genes. With two promoters associated with different exons, the myosin light-chain 1/3 gene generates two pre-mRNAs that also differ by the use of a pair of internal exons, 3 and 4, that are spliced in a mutually exclusive fashion. When the promoter upstream from exon 1 is used, only exon 4 is included. If the promoter upstream from exon 2 is used, only exon 3 is included. In an attempt to understand the molecular basis for the mutually exclusive behavior of these two exons and the basis of their specific selection, a number of minigene constructs containing exons 3 and 4 were tested in a variety of homologous or heterologous cis and trans environments. The results demonstrate that the mutually exclusive behavior of myosin light-chain exons 3 and 4 and selection between the two exons are cis regulated and are affected by the nature of the flanking sequences. Both exons competed for the common flanking 5' and 3' splice sites. Flanking exons were found that favored inclusion into mature mRNA of exon 3, exon 4, both, or neither, suggesting a specific cooperative interaction between certain 5' and 3' splice sites. Thus, alternative splicing of myosin light-chain 1/3 pre-mRNAs is regulated in cis by a hierarchy of compatibilities between pairs of 5' and 3' splice sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.