ArticleBioanalysis2013
Heat stabilization of blood spot samples for determination of metabolically unstable drug compounds.
Article in Bioanalysis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Pharmacokinetics and Dose Proportionality Study of a Novel Antiparkinsonian Agent, a 1Molecules (Basel, Switzerland) · 2024Article
- Protective mechanism of dried blood spheroids: stabilization of labile analytes in whole blood, plasma, and serum.The Analyst · 2021Article
- Evolution of the liver biopsy and its future.Translational gastroenterology and hepatology · 2021Review
- Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues.Scientific reports · 2018Article
- Stable-Isotope Dilution HPLC-Electrospray Ionization Tandem Mass Spectrometry Method for Quantifying Hydroxyurea in Dried Blood Samples.Clinical chemistry · 2016Article
- Clinical chemistry and dried blood spots: increasing laboratory utilization by improved understanding of quantitative challenges.Bioanalysis · 2014Article
- Pharmacokinetic studies in infants using minimal-risk study designs.Current clinical pharmacology · 2014Review
- Determination of artemether and dihydroartemisinin in human plasma with a new hydrogen peroxide stabilization method.Bioanalysis · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundSample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations.
resultsA novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60°C.
conclusionHeat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation.
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Registered trials
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