ArticleProceedings of the National Academy of Sciences of the United States of America2013
Intramolecular arrangement of sensor and regulator overcomes relaxed specificity in hybrid two-component systems.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
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- Structural features discriminating hybrid histidine kinase Rec domains from response regulator homologs.Nature communications · 2023Article
- Harnessing gut microbes for glycan detection and quantification.Nature communications · 2023Article
- Comparative Proteomic Analysis of Fucosylated Glycoproteins Produced byFrontiers in microbiology · 2022Article
- A Radical Reimagining of Fungal Two-Component Regulatory Systems.Trends in microbiology · 2021Review
- Evolutionary history expands the range of signaling interactions in hybrid multikinase networks.Scientific reports · 2021Article
- Hybrid histidine kinase activation by cyclic di-GMP-mediated domain liberation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- We're in this Together: Sensation of the Host Cell Environment by Endosymbiotic Bacteria.Results and problems in cell differentiation · 2020Article
- Engineering dual-glycan responsive expression systems for tunable production of heterologous proteins in Bacteroides thetaiotaomicron.Scientific reports · 2019Article
- Dietary sugar silences a colonization factor in a mammalian gut symbiont.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- An insider's perspective: Bacteroides as a window into the microbiome.Nature microbiology · 2017Review
- Degradation of cyclic diguanosine monophosphate by a hybrid two-component protein protects Azoarcus sp. strain CIB from toluene toxicity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Bacterial protein networks: properties and functions.Nature reviews. Microbiology · 2015Review
- Temporal and evolutionary dynamics of two-component signaling pathways.Current opinion in microbiology · 2015Review
- Toward rationally redesigning bacterial two-component signaling systems using coevolutionary information.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Phosphate flow between hybrid histidine kinases CheA₃ and CheS₃ controls Rhodospirillum centenum cyst formation.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Cellular processes require specific interactions between cognate protein partners and concomitant discrimination against noncognate partners. Signal transduction by classical two-component regulatory systems typically entails an intermolecular phosphoryl transfer between a sensor kinase (SK) and a cognate response regulator (RR). Interactions between noncognate partners are rare because SK/RR pairs coevolve unique interfaces that dictate phosphotransfer specificity. Here we report that the in vitro phosphotransfer specificity is relaxed in hybrid two-component systems (HTCSs) from the human gut symbiont Bacteroides thetaiotaomicron, which harbor both the SK and RR in a single polypeptide. In contrast, phosphotransfer specificity is retained in classical two-component regulatory systems from this organism. This relaxed specificity enabled us to rewire a HTCS successfully to transduce signals between noncognate SK/RR pairs. Despite the relaxed specificity between SK and RRs, HTCSs remained insulated from cross-talk with noncognate proteins in vivo. Our data suggest that the high local concentration of the SK and RR present in the same polypeptide maintains specificity while relaxing the constraints on coevolving unique contact interfaces.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.