ArticleThe Journal of general virology2013
Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe.
Article in The Journal of general virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 67 citations in OpenAlex.
- Conserved non-coding RNA motifs influence the neuropathogenicity of Simbuviruses: Molecular dissection in the Schmallenberg virus model.PLoS pathogens · 2026Article
- Oropouche Virus: An Emerging Arboviral Threat and Its Implications for Europe.Life (Basel, Switzerland) · 2025Review
- Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system.BMC veterinary research · 2025Article
- Oropouche Virus: An Emerging Orthobunyavirus.The Journal of general virology · 2024Review
- Bioluminescent and fluorescent reporter-expressing recombinant Akabane virus (AKAV): an excellent tool to dissect viral replication.Frontiers in microbiology · 2024Article
- Differential role of NSs genes in the neurovirulence of two genogroups of Akabane virus causing postnatal encephalomyelitis.Archives of virology · 2023Article
- Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion.Journal of virology · 2023Article
- Development of a Simian RNA Polymerase I Promoter-Driven Reverse Genetics for the Rescue of Recombinant Rift Valley Fever Virus from Vero Cells.Journal of virology · 2021Article
- Recent Advances in Bunyavirus Glycoprotein Research: Precursor Processing, Receptor Binding and Structure.Viruses · 2021Review
- Recent Advances in Bunyavirus Reverse Genetics Research: Systems Development, Applications, and Future Perspectives.Frontiers in microbiology · 2021Review
- Development of Rice Stripe Tenuivirus Minireplicon Reverse Genetics Systems Suitable for Analyses of Viral Replication and Intercellular Movement.Frontiers in microbiology · 2021Article
- Reverse Genetics System for Shuni Virus, an Emerging Orthobunyavirus with Zoonotic Potential.Viruses · 2020Article
- Review
- Immunogenicity and efficacy of Schmallenberg virus envelope glycoprotein subunit vaccines.Journal of veterinary science · 2019Article
- Schmallenberg virus: a systematic international literature review (2011-2019) from an Irish perspective.Irish veterinary journal · 2019Review
- Development of reverse genetics systems and investigation of host response antagonism and reassortment potential for Cache Valley and Kairi viruses, two emerging orthobunyaviruses of the Americas.PLoS neglected tropical diseases · 2018Article
- Mapping of Transcription Termination within the S Segment of SFTS Phlebovirus Facilitated Generation of NSs Deletant Viruses.Journal of virology · 2017Article
- Article
- Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus.Virology journal · 2017Article
- Nonstructural Protein NSs of Schmallenberg Virus Is Targeted to the Nucleolus and Induces Nucleolar Disorganization.Journal of virology · 2017Article
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8 authors at 1 institution in 1 country.
Funding
Abstract
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus that has caused widespread disease in cattle, sheep and goats in Europe. Like other orthobunyaviruses, SBV is characterized by a tripartite negative-sense RNA genome that encodes four structural and two non-structural proteins. This study showed that SBV has a wide in vitro host range, and that BHK-21 cells are a convenient host for both SBV propagation and assay by plaque titration. The SBV genome segments were cloned as cDNA and a three-plasmid rescue system was established to recover infectious virus. Recombinant virus behaved similarly in cell culture to authentic virus. The ORF for the non-structural NSs protein, encoded on the smallest genome segment, was disrupted by introduction of translation stop codons in the appropriate cDNA, and when this plasmid was used in reverse genetics, a recombinant virus that lacked NSs expression was recovered. This virus had reduced capacity to shut-off host-cell protein synthesis compared with the wild-type virus. In addition, the NSs-deleted virus induced interferon (IFN) in cells, indicating that, like other orthobunyaviruses, NSs functions as an IFN antagonist, most probably by globally inhibiting host-cell metabolism. The development of a robust reverse genetics system for SBV will facilitate investigation of its pathogenic mechanisms as well as the creation of attenuated strains that could be candidate vaccines.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.