ArticleMolecular biology reports2013
Oct4 and the small molecule inhibitor, SC1, regulates Tet2 expression in mouse embryonic stem cells.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Systematic identification of Oct4 transcriptional targets in embryonic stem cells using the auxin-inducible degron system and nascent RNA sequencing.Cell regeneration (London, England) · 2025Article
- DNA Methylation: A Key Regulator in Male and Female Reproductive Outcomes.Life (Basel, Switzerland) · 2025Review
- Ten-Eleven Translocation Family Proteins: Structure, Biological Functions, Diseases, and Targeted Therapy.MedComm · 2025Review
- Optimising Electroporation Condition for CRISPR/Cas-Mediated Knockout in Zona-Intact Buffalo Zygotes.Animals : an open access journal from MDPI · 2023Article
- Inhibition of DNA methylation attenuates lung ischemia-reperfusion injury after lung transplantation.The Journal of international medical research · 2023Article
- Mechanisms that regulate the activities of TET proteins.Cellular and molecular life sciences : CMLS · 2022Review
- Epigenetic cell memory: The gene's inner chromatin modification circuit.PLoS computational biology · 2022Article
- TETology: Epigenetic Mastermind in Action.Applied biochemistry and biotechnology · 2021Review
- The Role of Host Cell DNA Methylation in the Immune Response to Bacterial Infection.Frontiers in immunology · 2021Review
- The roles of TET family proteins in development and stem cells.Development (Cambridge, England) · 2020Review
- The Complexity of TET2 Functions in Pluripotency and Development.Frontiers in cell and developmental biology · 2020Review
- The histone deacetylase SIRT6 controls embryonic stem cell fate via TET-mediated production of 5-hydroxymethylcytosine.Nature cell biology · 2015Article
- Dynamic switching of active promoter and enhancer domains regulates Tet1 and Tet2 expression during cell state transitions between pluripotency and differentiation.Molecular and cellular biology · 2015Article
- Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human.PloS one · 2015Article
- The Ten-Eleven Translocation-2 (TET2) gene in hematopoiesis and hematopoietic diseases.Leukemia · 2014Review
- An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis.Cell reports · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ten eleven translocation (Tet) family of proteins includes three members (Tet1-3), all of which have the capacity to convert 5-methylcytosine to 5-hydroxymethylcytosine in a 2-oxoglutarate- and Fe(II)-dependent manner. Tet1 and Tet2 are highly expressed in undifferentiated embryonic stem cells (ESCs), and this expression decreases upon differentiation. Notably, the expression patterns of Tet1 and Tet2 in ESCs parallels that of pluripotency genes. To date, however, the mechanisms underlying the regulation of Tet gene expression in ESCs remain largely unexplored. Here we report that the pluripotency transcription factor, Oct4, directly regulates the expression of Tet2. Using RNAi, real time quantitative PCR, dual-luciferase reporter assays and electrophoretic mobility shift assays, we show that Oct4 promotes Tet2 transcription by binding to consensus sites in the proximal promoter region. Furthermore, we explored the role of the small molecule inhibitor, SC1 (pluripotin) on Tet gene expression. We show that SC1 promotes Tet3 expression, but represses Tet1 and Tet2 expression. Our findings indicate that Tet2 are crucial downstream targets of the pluripotency factor Oct4, and highlight a role for Oct4 in the regulation of DNA methylation in ESCs. In addition, these findings also provide a new insight into drug-mediated gene regulation.
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