ArticleJournal of synchrotron radiation2013
Global radiation damage: temperature dependence, time dependence and how to outrun it.
Article in Journal of synchrotron radiation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Risk of secondary rectal cancer and colon cancer after radiotherapy for prostate cancer: a meta-analysis.International journal of colorectal disease · 2018Pooled it
- Separating the effects of temperature and absorbed X-ray dose on unit-cell volume.Acta crystallographica. Section D, Structural biology · 2026Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- Room-temperature serial synchrotron crystallography of the human phosphatase PTP1B.Acta crystallographica. Section F, Structural biology communications · 2023Article
- Evaluating the impact of X-ray damage on conformational heterogeneity in room-temperature (277 K) and cryo-cooled protein crystals.Acta crystallographica. Section D, Structural biology · 2022Article
- FMX - the Frontier Microfocusing Macromolecular Crystallography Beamline at the National Synchrotron Light Source II.Journal of synchrotron radiation · 2021Article
- Serial Crystallography for Structure-Based Drug Discovery.Trends in pharmacological sciences · 2020Review
- Solvent flows, conformation changes and lattice reordering in a cold protein crystal.Acta crystallographica. Section D, Structural biology · 2019Article
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- Sample Delivery Media for Serial Crystallography.International journal of molecular sciences · 2019Review
- Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography.Acta crystallographica. Section D, Structural biology · 2019Article
- Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.Acta crystallographica. Section D, Structural biology · 2019Article
- High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner.Journal of synchrotron radiation · 2018Article
- Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.Structure (London, England : 1993) · 2018Article
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- Watching Proteins Function with Time-resolved X-ray Crystallography.Journal of physics D: Applied physics · 2017Article
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Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A series of studies that provide a consistent and illuminating picture of global radiation damage to protein crystals, especially at temperatures above ∼200 K, are described. The radiation sensitivity shows a transition near 200 K, above which it appears to be limited by solvent-coupled diffusive processes. Consistent with this interpretation, a component of global damage proceeds on timescales of several minutes at 180 K, decreasing to seconds near room temperature. As a result, data collection times of order 1 s allow up to half of global damage to be outrun at 260 K. Much larger damage reductions near room temperature should be feasible using larger dose rates delivered using microfocused beams, enabling a significant expansion of structural studies of proteins under more nearly native conditions.
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Registered trials
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