ArticleInternational journal of legal medicine2013
miRNA analysis in vitreous humor to determine the time of death: a proof-of-concept pilot study.
Article in International journal of legal medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 3 syntheses or guidelines pooled it, 55 citations in OpenAlex.
- microRNAs as New Biomolecular Markers to Estimate Time since Death: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- A small RNA, microRNA as a potential biomolecular marker to estimate post mortem interval in forensic science: a systematic review.International journal of legal medicine · 2023Pooled it
- Acute Spinal Cord Injury: A Systematic Review Investigating miRNA Families Involved.International journal of molecular sciences · 2019Pooled it
- Estimating time of day from fingertip blood samples using RNA molecules with diurnal oscillating expression: a proof-of-principle study.International journal of legal medicine · 2026Article
- Selection and Validation of Endogenous Reference microRNAs for Post-Mortem Interval Estimation in Vitreous Humor: A Preliminary Study.International journal of molecular sciences · 2026Article
- Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals.Non-coding RNA · 2024Article
- MiR-122, miR-133a, and miR-206 as potential biomarkers for post-mortem interval estimation.Genes & genomics · 2024Article
- The potential of using non-coding RNAs in forensic science applications.Forensic sciences research · 2023Review
- Common Ground between Biological Rhythms and Forensics.Biology · 2022Review
- Review
- MicroRNAs as Useful Tools to Estimate Time Since Death. A Systematic Review of Current Literature.Diagnostics (Basel, Switzerland) · 2021Review
- MicroRNAs: An Update of Applications in Forensic Science.Diagnostics (Basel, Switzerland) · 2020Review
- Biological Involvement of MicroRNAs in Proliferative Vitreoretinopathy.Translational vision science & technology · 2017Review
- Methods for determining time of death.Forensic science, medicine, and pathology · 2016Article
- Exploration of the R code-based mathematical model for PMI estimation using profiling of RNA degradation in rat brain tissue at different temperatures.Forensic science, medicine, and pathology · 2015Article
- miR-888: A Novel Cancer-Testis Antigen that Targets the Progesterone Receptor in Endometrial Cancer.Translational oncology · 2015Article
- Molecular analysis of different classes of RNA molecules from formalin-fixed paraffin-embedded autoptic tissues: a pilot study.International journal of legal medicine · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We hypothesized that miRNAs present in vitreous humor could be a sort of "biological black box," storing information about physiological and environmental circumstances at death. As a proof of concept, we analyzed the vitreous humor miRNA signature to explore its forensic potential applications, such as determining the time of the day at death. The miRNAs present in vitreous humor from individuals who died at daytime or at nighttime were analyzed by quantitative real-time polymerase chain reaction (qPCR) array. Target miRNAs showing significant differences between groups were studied in a larger sample by individual qPCR assays. After array analysis of miRNAs in seven samples, significant expression differences were detected between individuals who died at daytime and at nighttime regarding mir-34c, mir-541, mir-888, mir-484, and mir-142-5p. miR-222 appeared as the best reference gene. The results were replicated in 34 vitreous humor samples, and the day-night differences were confirmed for miR-142-5p and miR-541, suggesting that miRNA levels may be related to either the ambient light or the circadian clock at the time of death. There was no correlation between miRNA levels and the time elapsed after death, suggesting that they were stable at least for 24 h. In conclusion, this report supports the potential forensic utility of the analysis of miRNAs in the vitreous humor in applications such as determining the time of death.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.