ArticleJournal of assisted reproduction and genetics2013
False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3.
Article in Journal of assisted reproduction and genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Can Comprehensive Chromosome Screening Technology Improve IVF/ICSI Outcomes? A Meta-Analysis.PloS one · 2015Pooled it
- Preimplantation Genetic Screening with Spent Culture Medium/Blastocoel Fluid for in Vitro Fertilization.Scientific reports · 2018Article
- New Promising Strategies in Oncofertility.Expert review of quality of life in cancer care · 2017Article
- What Drives Embryo Development? Chromosomal Normality or Mitochondria?Case reports in genetics · 2017Article
- Confirmation rates of array-CGH in day-3 embryo and blastocyst biopsies for preimplantation genetic screening.Journal of assisted reproduction and genetics · 2016Article
- Comparison of array comparative genomic hybridization and quantitative real-time PCR-based aneuploidy screening of blastocyst biopsies.European journal of human genetics : EJHG · 2015Observational
- New tools for embryo selection: comprehensive chromosome screening by array comparative genomic hybridization.BioMed research international · 2014Article
- Clinical outcome of preimplantation genetic diagnosis and screening using next generation sequencing.GigaScience · 2014Article
- Embryonic Aneuploidy after Preimplantation Genetic Screening: Age- and Indication-Matched Comparative Study between Indian and Spanish Population.Journal of human reproductive sciencesArticle
- Morphokinetic behavior of euploid and aneuploid embryos analyzed by time-lapse in embryoscope.Journal of human reproductive sciencesArticle
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, false positive rate of an arrayCGH platform for its use in day-3 single-blastomere analysis was calculated. For this purpose, 38 embryos diagnosed as abnormal on day-3 by FISH were re-biopsied on day-4. Single-cell day-4 arrayCGH diagnosis was then performed. A successful amplification was obtained in 97.4 % (37/38) of the day-4 cells analysed by arrayCGH. Day-3 FISH and day-4 arrayCGH diagnosis were concordant in 35/37 cases. The two discordant embryos were spread and all the cells from each embryo were re-analysed by FISH on day 5. The same error rate (2.7 %) for day-3 FISH and day-4 arrayCGH was obtained when comparing day-5 FISH re-analysis. After this pre-clinical phase, the platform was used for day-3 arrayCGH clinical application in 320 patients (1,760 embryos). Day-3 amplification rate was 98.6 %. An optimal reproductive outcome was obtained when applying arrayCGH to a clinical program: clinical pregnancy rate per cycle of 38.4 % and 60.3 % per transference were obtained, with an implantation rate of 53.5 %. Overall miscarriage rate was 10.6 %. Additionally, day-5 FISH re-analysis was performed in 42 of the embryos from the clinical phase, obtaining a concordance rate of 97.6 % with day-3 arrayCGH.
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