ArticleBMC genomics2012
Differences in enhancer activity in mouse and zebrafish reporter assays are often associated with changes in gene expression.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Modeling myeloid cell development in health and disease using induced pluripotent stem cells.Frontiers in immunology · 2026Review
- Biocompatibility Analysis of Bio-Based and Synthetic Silica Nanoparticles during Early Zebrafish Development.International journal of molecular sciences · 2024Article
- Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers.Nature communications · 2022Article
- Article
- Identification and functional characterization of novel transcriptional enhancers involved in regulating human GLI3 expression during early development.Development, growth & differentiation · 2015Article
- Review
- Article
- Enhancer turnover and conserved regulatory function in vertebrate evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Deep conservation of cis-regulatory elements in metazoans.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Functional analysis of limb enhancers in the developing fin.Development genes and evolution · 2013Article
- A novel test for selection on cis-regulatory elements reveals positive and negative selection acting on mammalian transcriptional enhancers.Molecular biology and evolution · 2013Article
- Exaptation of transposable elements into novel cis-regulatory elements: is the evidence always strong?Molecular biology and evolution · 2013Review
Corrections and comments
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Authors and funding
7 authors at 7 institutions in 3 countries.
Funding
Abstract
backgroundPhenotypic evolution in animals is thought to be driven in large part by differences in gene expression patterns, which can result from sequence changes in cis-regulatory elements (cis-changes) or from changes in the expression pattern or function of transcription factors (trans-changes). While isolated examples of trans-changes have been identified, the scale of their overall contribution to regulatory and phenotypic evolution remains unclear.
resultsHere, we attempt to examine the prevalence of trans-effects and their potential impact on gene expression patterns in vertebrate evolution by comparing the function of identical human tissue-specific enhancer sequences in two highly divergent vertebrate model systems, mouse and zebrafish. Among 47 human conserved non-coding elements (CNEs) tested in transgenic mouse embryos and in stable zebrafish lines, at least one species-specific expression domain was observed in the majority (83%) of cases, and 36% presented dramatically different expression patterns between the two species. Although some of these discrepancies may be due to the use of different transgenesis systems in mouse and zebrafish, in some instances we found an association between differences in enhancer activity and changes in the endogenous gene expression patterns between mouse and zebrafish, suggesting a potential role for trans-changes in the evolution of gene expression.
conclusionsIn total, our results: (i) serve as a cautionary tale for studies investigating the role of human enhancers in different model organisms, and (ii) suggest that changes in the trans environment may play a significant role in the evolution of gene expression in vertebrates.
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