ArticleBioconjugate chemistry2013
Direct detection of collagenous proteins by fluorescently labeled collagen mimetic peptides.
Article in Bioconjugate chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 103 citations in OpenAlex.
- Differences in collagen structure and organization in primary and metastatic pancreatic cancer and the effects of FOLFIRINOX treatment.Matrix biology plus · 2026Article
- Collagen hybridizing peptide to target in vivo misfolded collagen in OI zebrafish.Scientific reports · 2026Article
- Biomimetic peptide conjugates as emerging strategies for controlled release from protein-based materials.Drug delivery · 2025Review
- A Novel Approach for Optimizing Molecularly Imprinted Polymer Composition in Electrochemical Detection of Collagen Peptides.Bioengineering (Basel, Switzerland) · 2025Article
- Synovial matrix turnover controls immune cell spatial patterning in inflammation resolution.Molecular systems biology · 2025Article
- Around the collagen triple helix: an introduction to studying associated genetic and acquired diseases.Matrix biology : journal of the International Society for Matrix Biology · 2025Review
- Fluorescent collagen hybridizing peptide for quantifying collagen denaturation in cortical bone.Bone reports · 2025Article
- Topical Application of a Collagen Mimetic Peptide Restores Peripapillary Scleral Stiffness Reduced by Ocular Stress.Pharmaceuticals (Basel, Switzerland) · 2025Article
- The staphylococcal collagen adhesin CNA35 effectively detects collagen and its fragments in blots after SDS-PAGE.Matrix biology plus · 2025Article
- Collagen Hybridizing Peptide-Based Radiotracers for Molecular Imaging of Collagen Turnover in Pulmonary Fibrosis.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2025Article
- Collagen integrity of the annulus fibrosus in degenerative disc disease individuals quantified with collagen hybridizing peptide.JOR spine · 2024Article
- From Collagen Mimetics to Collagen Hybridization and Back.Accounts of chemical research · 2024Article
- Simultaneous fingerprinting of multiplex collagen biomarkers in connective tissues by multicolor quantum dots-based peptide probes.Materials today. Bio · 2024Article
- Targeting damaged collagen for intra-articular delivery of therapeutics using collagen hybridizing peptides.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Targeting Peptides: The New Generation of Targeted Drug Delivery Systems.Pharmaceutics · 2023Review
- The Chemistry and Biology of Collagen Hybridization.Journal of the American Chemical Society · 2023Review
- Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development.Journal of cardiovascular translational research · 2023Article
- Mouse Achilles tendons exhibit collagen disorganization but minimal collagen denaturation during cyclic loading to failure.Journal of biomechanics · 2023Article
- Collagen fibrils from both positional and energy-storing tendons exhibit increased amounts of denatured collagen when stretched beyond the yield point.Acta biomaterialia · 2023Article
- Perturbations in fatty acid metabolism and collagen production infer pathogenicity of a novelFrontiers in endocrinology · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Although fibrous collagens are major structural components of extracellular matrix in mammals, collagen overproduction is associated with many human diseases including cancers and fibrosis. Collagen is typically identified in biomedical research by Western blot and immunohistochemistry; however, anticollagen antibodies employed in these analyses are difficult to prepare and their affinities to collagen can diminish if collagen becomes denatured during analyses. Previously, we discovered that single-stranded collagen mimetic peptides [CMPs, sequence: (GlyProHyp)(9)] can bind to denatured collagen chains by triple helix hybridization. Here, we present collagen-specific staining methods using simple CMPs conjugated to common fluorophores (e.g., carboxyfluorescein), which allow direct detection of collagens and collagen-like proteins in SDS-PAGE and in various mammalian tissue sections. By directly staining SDS-PAGE gels with fluorescently labeled CMPs, both intact (type I, II, and IV) and MMP-1 cleaved collagen (type I) chains as well as complement factor C1q were detected. Collagen bands containing as little as 5 ng were optically visualized, while no staining was observed for fibronectin, laminin, and a collection of proteins from mammalian cell lysate. The CMP was unable to stain collagen-like bacterial protein, which contains numerous charged amino acids that are believed to stabilize triple helix in place of Hyp. We also show that fluorescently labeled CMPs can specifically visualize collagens in fixed tissue sections (e.g., skin, cornea, and bone) more effectively than anticollagen I antibody, and allow facile identification of pathologic conditions in fibrotic liver tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.