ArticleNeurogastroenterology and motility2013
Visualization of spinal afferent innervation in the mouse colon by AAV8-mediated GFP expression.
Article in Neurogastroenterology and motility, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Gut-brain communication: types of sensory nerves and mechanisms of activation.Nature reviews. Gastroenterology & hepatology · 2026Review
- DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.Cell · 2023Article
- AAV-mediated gene transfer to colon-innervating primary afferent neurons.Frontiers in pain research (Lausanne, Switzerland) · 2023Article
- Reduced activity of adenylyl cyclase 1 attenuates morphine induced hyperalgesia and inflammatory pain in mice.Frontiers in pharmacology · 2022Article
- Modulation of SUR1 KInternational journal of molecular sciences · 2019Article
- Morphine Efficacy, Tolerance, and Hypersensitivity Are Altered After Modulation of SUR1 Subtype KFrontiers in neuroscience · 2019Article
- AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Optogenetic and chemogenetic techniques for neurogastroenterology.Nature reviews. Gastroenterology & hepatology · 2018Review
- ImprovedMolecular therapy. Methods & clinical development · 2017Article
- Targeting the gastrointestinal tract with viral vectors: state of the art and possible applications in research and therapy.Histochemistry and cell biology · 2016Review
- TRP channel functions in the gastrointestinal tract.Seminars in immunopathology · 2016Review
- Adeno-Associated Viral Vector Delivery to the Enteric Nervous System: A Review.Postdoc journal : a journal of postdoctoral research and postdoctoral affairs · 2015Article
- Overexpression of Sox11 promotes corticospinal tract regeneration after spinal injury while interfering with functional recovery.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Distribution across tissue layers of extrinsic nerves innervating the mouse colorectum - an in vitro anterograde tracing study.Neurogastroenterology and motility · 2014Article
- Biodistribution of adeno-associated virus serotype 9 (AAV9) vector after intrathecal and intravenous delivery in mouse.Frontiers in neuroanatomy · 2014Article
- Molecular anatomy of the gut-brain axis revealed with transgenic technologies: implications in metabolic research.Frontiers in neuroscience · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPrimary afferent neurons whose cell bodies reside in thoracolumbar and lumbosacral dorsal root ganglia (DRG) innervate colon and transmit sensory signals from colon to spinal cord under normal conditions and conditions of visceral hypersensitivity. Histologically, these extrinsic afferents cannot be differentiated from intrinsic fibers of enteric neurons because all known markers label neurons of both populations. Adeno-associated virus (AAV) vectors are capable of transducing DRG neurons after intrathecal administration. We hypothesized that AAV-driven overexpression of green fluorescent protein (GFP) in DRG would enable visualization of extrinsic spinal afferents in colon separately from enteric neurons.
methodsRecombinant AAV serotype 8 (rAAV8) vector carrying the GFP gene was delivered via direct lumbar puncture. Green fluorescent protein labeling in DRG and colon was examined using immunohistochemistry. KEY
resultsAnalysis of colon from rAAV8-GFP-treated mice demonstrated GFP-immunoreactivity (GFP-ir) within mesenteric nerves, smooth muscle layers, myenteric plexus, submucosa, and mucosa, but not in cell bodies of enteric neurons. Notably, GFP-ir colocalized with CGRP and TRPV1 in mucosa, myenteric plexus, and globular-like clusters surrounding nuclei within myenteric ganglia. In addition, GFP-positive fibers were observed in close association with blood vessels of mucosa and submucosa. Analysis of GFP-ir in thoracolumbar and lumbosacral DRG revealed that levels of expression in colon and L6 DRG appeared to be related. CONCLUSIONS & INFERENCES: These results demonstrate the feasibility of gene transfer to mouse colonic spinal sensory neurons using intrathecal delivery of AAV vectors and the utility of this approach for histological analysis of spinal afferent nerve fibers within colon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.