ArticleScientific reports2012
Stability of miRNA 5'terminal and seed regions is correlated with experimentally observed miRNA-mediated silencing efficacy.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Profile of miRNA expression in the hippocampus of epileptic mice and the prediction of potential therapeutic targets.Molecular biology reports · 2024Article
- Early Leishmania infectivity depends on miR-372/373/520d family-mediated reprogramming of polyamines metabolism in THP-1-derived macrophages.Scientific reports · 2024Article
- sRNAfrag: a pipeline and suite of tools to analyze fragmentation in small RNA sequencing data.Briefings in bioinformatics · 2023Article
- Turning off a few overexpressed genes in prostate cancer with microRNAs using a 7mer-seed match model.Journal of cancer research and clinical oncology · 2023Article
- Structural Insights into the Advances and Mechanistic Understanding of Human Dicer.Biochemistry · 2023Review
- The Four mRNA Bases Have Quite Different (Un)folding Free Energies, Applications to RNA Splicing and Translation Initiation with BindOligoNet.Journal of molecular biology · 2022Article
- EpisomiR, a New Family of miRNAs, and Its Possible Roles in Human Diseases.Biomedicines · 2022Review
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- Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses.Scientific reports · 2021Article
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- A robust model for quantitative prediction of the silencing efficacy of wild-type and A-to-I edited miRNAs.RNA biology · 2020Article
- MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells.Frontiers in cell and developmental biology · 2020Article
- Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito,Viruses · 2019Article
- The sequence features that define efficient and specific hAGO2-dependent miRNA silencing guides.Nucleic acids research · 2018Article
- Base-pairing probability in the microRNA stem region affects the binding and editing specificity of human A-to-I editing enzymes ADAR1-p110 and ADAR2.RNA biology · 2018Article
- On revealing the gene targets of Ebola virus microRNAs involved in the human skin microbiome.PeerJ · 2018Article
- miR-141 promotes colon cancer cell proliferation by inhibiting MAP2K4.Oncology letters · 2017Article
- Large-scale analysis of microRNA expression, epi-transcriptomic features and biogenesis.Nucleic acids research · 2017Article
- Article
- Terminal Duplex Stability and Nucleotide Identity Differentially Control siRNA Loading and Activity in RNA Interference.Nucleic acid therapeutics · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are key regulators of sequence-specific gene silencing. However, crucial factors that determine the efficacy of miRNA-mediated target gene silencing are poorly understood. Here we mathematized base-pairing stability and showed that miRNAs with an unstable 5' terminal duplex and stable seed-target duplex exhibit strong silencing activity. The results are consistent with the previous findings that an RNA strand with unstable 5' terminal in miRNA duplex easily loads onto the RNA-induced silencing complex (RISC), and miRNA recognizes target mRNAs with seed-complementary sequences to direct posttranscriptional repression. Our results suggested that both the unwinding and target recognition processes of miRNAs could be proficiently controlled by the thermodynamics of base-pairing in protein-free condition. Interestingly, such thermodynamic parameters might be evolutionarily well adapted to the body temperatures of various species.
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