ArticlePloS one2012
Activity of 25-hydroxylase in human gingival fibroblasts and periodontal ligament cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Vitamin D as a candidate host susceptibility factor in sugar-driven oral dysbiosis.Frontiers in oral health · 2026Pooled it
- Evaluation of Vitamin D and of Some Biomarkers of Bone Remodelling (CTX-1, Osteocalcin, BALP) in Subjects with Periapical Inflammatory Cysts: An Observational Study.Journal of clinical medicine · 2025Article
- High-Throughput Combined Analysis of Saliva Microbiota and Metabolomic Profile in Chinese Periodontitis Patients: A Pilot Study.Inflammation · 2024Article
- 1,25(OH)2D3 increase osteogenic potential of human periodontal ligament cells with low osteoblast potential.Journal of applied oral science : revista FOB · 2024Article
- 25-hydroxyvitamin DFrontiers in immunology · 2023Article
- Osteoporosis and Alveolar Bone Health in Periodontitis Niche: A Predisposing Factors-Centered Review.Cells · 2022Review
- The role of 25-hydroxyvitamin-D3 and vitamin D receptor gene in human periodontal ligament fibroblasts as response to orthodontic compressive strain: an in vitro study.BMC oral health · 2021Article
- Effect of vitamin DJournal of periodontal research · 2021Article
- Transcriptional activity of vitamin D receptor in human periodontal ligament cells is diminished under inflammatory conditions.Journal of periodontology · 2021Article
- Article
- Pleiotropic effects of vitamin DJournal of clinical periodontology · 2020Article
- Could Vitamin D influence risk for Periodontal Disease - to "D" or not to "D"?Current oral health reports · 2020Article
- 1,25(OH)Journal of clinical medicine · 2019Article
- Ferritin expression in the periodontal tissues of primates.European journal of histochemistry : EJH · 2019Article
- The Relationship between Vitamin D and Periodontal Pathology.Medicina (Kaunas, Lithuania) · 2018Review
- Both 25-hydroxyvitamin-D3 and 1,25-dihydroxyvitamin-D3 reduces inflammatory response in human periodontal ligament cells.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe previously demonstrated that 25-hydroxyvitamin D(3) concentrations in gingival crevicular fluid are 300 times higher than those in the plasma of patients with aggressive periodontitis. Here we explored whether 25-hydroxyvitamin D(3) can be synthesized by periodontal soft tissue cells. We also investigated which of the two main kinds of hydroxylases, CYP27A1 and CYP2R1, is the key 25-hydroxylase in periodontal soft tissue cells. METHODOLOGY/PRINCIPAL
findingsPrimary cultures of human gingival fibroblasts and periodontal ligament cells from 5 individual donors were established. CYP27A1 mRNA, CYP2R1 mRNA and CYP27A1 protein were detected in human gingival fibroblasts and periodontal ligament cells, whereas CYP2R1 protein was not. After incubation with the 25-hydroxylase substrate vitamin D(3), human gingival fibroblasts and periodontal ligament cells generated detectable 25-hydroxyvitamin D(3) that resulted in the production of 1α,25-dihydroxyvitamin D(3). Specific knockdown of CYP27A1 in human gingival fibroblasts and periodontal ligament cells using siRNA resulted in a significant reduction in both 25-hydroxyvitamin D(3) and 1α,25-dihydroxyvitamin D(3) production. Knockdown of CYP2R1 did not significantly influence 25-hydroxyvitamin D(3) synthesis. Sodium butyrate did not influence significantly CYP27A1 mRNA expression; however, interleukin-1β and Porphyromonas gingivalis lipopolysaccharide strongly induced CYP27A1 mRNA expression in human gingival fibroblasts and periodontal ligament cells.
conclusionsThe activity of 25-hydroxylase was verified in human gingival fibroblasts and periodontal ligament cells, and CYP27A1 was identified as the key 25-hydroxylase in these cells.
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