ArticlePloS one2012
Two types of Tet-On transgenic lines for doxycycline-inducible gene expression in zebrafish rod photoreceptors and a gateway-based tet-on toolkit.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
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- Abca4, mutated in Stargardt disease, is required for structural integrity of cone outer segments.Disease models & mechanisms · 2025Article
- Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights.Frontiers in molecular neuroscience · 2024Review
- A scalable and tunable platform for functional interrogation of peptide hormones in fish.eLife · 2023Article
- Chimeric efferocytic receptors improve apoptotic cell clearance and alleviate inflammation.Cell · 2022Article
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- Inducible modulation of miR-204 levels in a zebrafish melanoma model.Biology open · 2020Article
- A comparison between chemical and gas hypoxia as models of global ischemia in zebrafish (Animal models and experimental medicine · 2020Article
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- A high content, small molecule screen identifies candidate molecular pathways that regulate rod photoreceptor outer segment renewal.Scientific reports · 2018Article
- Life-Long Neurogenic Activity of Individual Neural Stem Cells and Continuous Growth Establish an Outside-In Architecture in the Teleost Pallium.Current biology : CB · 2017Article
- Phosphodiesterase Inhibitors Sildenafil and Vardenafil Reduce Zebrafish Rod Photoreceptor Outer Segment Shedding.Investigative ophthalmology & visual science · 2017Article
- Neuropsychiatric Effects of Antimicrobial Agents.Clinical drug investigation · 2017Review
- Opportunities for CRISPR/Cas9 Gene Editing in Retinal Regeneration Research.Frontiers in cell and developmental biology · 2017Review
- Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain.Developmental biology · 2016Article
- Cichlid fishes as a model to understand normal and clinical craniofacial variation.Developmental biology · 2016Article
- A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.PloS one · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to control transgene expression within specific tissues is an important tool for studying the molecular and cellular mechanisms of development, physiology, and disease. We developed a Tet-On system for spatial and temporal control of transgene expression in zebrafish rod photoreceptors. We generated two transgenic lines using the Xenopus rhodopsin promoter to drive the reverse tetracycline-controlled transcriptional transactivator (rtTA), one with self-reporting GFP activity and one with an epitope tagged rtTA. The self-reporting line includes a tetracycline response element (TRE)-driven GFP and, in the presence of doxycycline, expresses GFP in larval and adult rods. A time-course of doxycycline treatment demonstrates that maximal induction of GFP expression, as determined by the number of GFP-positive rods, is reached within approximately 24 hours of drug treatment. The epitope-tagged transgenic line eliminates the need for the self-reporting GFP activity by expressing a FLAG-tagged rtTA protein. Both lines demonstrate strong induction of TRE-driven transgenes from plasmids microinjected into one-cell embryos. These results show that spatial and temporal control of transgene expression can be achieved in rod photoreceptors. Additionally, system components are constructed in Gateway compatible vectors for the rapid cloning of doxycycline-inducible transgenes and use in other areas of zebrafish research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.