ArticlePloS one2012
Time-lapse imaging of neuroblastoma cells to determine cell fate upon gene knockdown.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Regulatory Relationships between DNA Methylation and Long Noncoding RNAs in Neuroblastoma.Current medicinal chemistry · 2026Review
- Clinical significance of SNRPD1 expression in predicting post-transplant recurrence of hepatocellular carcinoma.American journal of translational research · 2026Article
- Complementary biomarkers of computed tomography for diagnostic grading of gastric cancer: DSCC1 and GINS1.Aging · 2024Article
- Decoding the DSCC1 gene as a pan-cancer biomarker in human cancers via comprehensive multi-omics analyses.American journal of translational research · 2024Article
- Significance of Spliceosome-Related Genes in the Prediction of Prognosis and Treatment Strategies for Lung Adenocarcinoma.BioMed research international · 2022Article
- The diagnostic and prognostic significance of small nuclear ribonucleoprotein Sm D1 aberrantly high expression in hepatocellular carcinoma.Journal of Cancer · 2022Article
- Prognostic prediction and gene regulation network ofJournal of gastrointestinal oncology · 2021Article
- High DSCC1 Level Predicts Poor Prognosis of Lung Adenocarcinoma.International journal of general medicine · 2021Article
- Single cell sequencing of radial glia progeny reveals the diversity of newborn neurons in the adult zebrafish brain.Development (Cambridge, England) · 2020Article
- DNA replication and sister chromatid cohesion 1 promotes breast carcinoma progression by modulating the Wnt/β-catenin signaling and p53 protein.Journal of biosciences · 2020Article
- Lymphovascular invasion and histologic grade are associated with specific genomic profiles in invasive carcinomas of the breast.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Counting unstained, confluent cells by modified bright-field microscopy.BioTechniques · 2013Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuroblastoma is the most common extra-cranial solid tumor of early childhood. Standard therapies are not effective in case of poor prognosis and chemotherapy resistance. To improve drug therapy, it is imperative to discover new targets that play a substantial role in tumorigenesis of neuroblastoma. The mitotic machinery is an attractive target for therapeutic interventions and inhibitors can be developed to target mitotic entry, spindle apparatus, spindle activation checkpoint, and mitotic exit. We present an elaborate analysis pipeline to determine cancer specific therapeutic targets by first performing a focused gene expression analysis to select genes followed by a gene knockdown screening assay of live cells. We interrogated gene expression studies of neuroblastoma tumors and selected 240 genes relevant for tumorigenesis and cell cycle. With these genes we performed time-lapse screening of gene knockdowns in neuroblastoma cells. We classified cellular phenotypes and used the temporal context of the perturbation effect to determine the sequence of events, particularly the mitotic entry preceding cell death. Based upon this phenotype kinetics from the gene knockdown screening, we inferred dynamic gene functions in mitosis and cell proliferation. We identified six genes (DLGAP5, DSCC1, SMO, SNRPD1, SSBP1, and UBE2C) with a vital role in mitosis and these are promising therapeutic targets for neuroblastoma. Images and movies of every time point of all screened genes are available at https://ichip.bioquant.uni-heidelberg.de.
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