ArticleThe Journal of biological chemistry2013
Self-assembly at air/water interfaces and carbohydrate binding properties of the small secreted protein EPL1 from the fungus Trichoderma atroviride.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 94 citations in OpenAlex.
- The Biological Roles of Class II Hydrophobins at the Cell Wall of Filamentous Ascomycetes.Journal of basic microbiology · 2026Review
- Inoculation withPlants (Basel, Switzerland) · 2025Article
- In Silico Evaluation, Phylogenetic Analysis, and Structural Modeling of the Class II Hydrophobin Family from Different Fungal Phytopathogens.Microorganisms · 2023Article
- Fused expression of Sm1-Chit42 proteins for synergistic mycoparasitic response of Trichoderma afroharzianum on Botrytis cinerea.Microbial cell factories · 2023Article
- The Cerato-Platanin EPL2 fromMicroorganisms · 2023Article
- Review
- Transcriptome Analysis ofBiology · 2022Article
- Metabolic Potential of Halophilic Filamentous Fungi-Current Perspective.International journal of molecular sciences · 2022Review
- At least three families of hyphosphere small secreted cysteine-rich proteins can optimize surface properties to a moderately hydrophilic state suitable for fungal attachment.Environmental microbiology · 2021Article
- Trichoderma harzianum cerato-platanin enhances hydrolysis of lignocellulosic materials.Microbial biotechnology · 2021Article
- The Evolutionary and Functional Paradox of Cerato-platanins in Fungi.Applied and environmental microbiology · 2020Article
- Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers.International journal of molecular sciences · 2020Article
- Characterization ofFrontiers in microbiology · 2020Article
- Mycelial form of dimorphic fungusIndian journal of microbiology · 2019Article
- A Cerato-Platanin Family Protein FocCP1 Is Essential for the Penetration and Virulence ofInternational journal of molecular sciences · 2019Article
- Functional analysis of eliciting plant response protein Epl1-Tas from Trichoderma asperellum ACCC30536.Scientific reports · 2018Article
- Necrotrophic Mycoparasites and Their Genomes.Microbiology spectrum · 2017Review
- TheFrontiers in plant science · 2017Article
- A single amino acid mutation affects elicitor and expansins-like activities of cerato-platanin, a non-catalytic fungal protein.PloS one · 2017Article
- Involvement ofFrontiers in plant science · 2017Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The protein EPL1 from the fungus Trichoderma atroviride belongs to the cerato-platanin protein family. These proteins occur only in filamentous fungi and are associated with the induction of defense responses in plants and allergic reactions in humans. However, fungi with other lifestyles also express cerato-platanin proteins, and the primary function of this protein family has not yet been elucidated. In this study, we investigated the biochemical properties of the cerato-platanin protein EPL1 from T. atroviride. Our results showed that EPL1 readily self-assembles at air/water interfaces and forms protein layers that can be redissolved in water. These properties are reminiscent of hydrophobins, which are amphiphilic fungal proteins that accumulate at interfaces. Atomic force microscopy imaging showed that EPL1 assembles into irregular meshwork-like substructures. Furthermore, surface activity measurements with EPL1 revealed that, in contrast to hydrophobins, EPL1 increases the polarity of aqueous solutions and surfaces. In addition, EPL1 was found to bind to various forms of polymeric chitin. The T. atroviride genome contains three epl genes. epl1 was predominantly expressed during hyphal growth, whereas epl2 was mainly expressed during spore formation, suggesting that the respective proteins are involved in different biological processes. For epl3, no gene expression was detected under most growth conditions. Single and double gene knock-out strains of epl1 and epl2 did not reveal a detectable phenotype, showing that these proteins are not essential for fungal growth and development despite their abundant expression.
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