ArticleCellular oncology (Dordrecht, Netherlands)2013
SLUG silencing increases radiosensitivity of melanoma cells in vitro.
Article in Cellular oncology (Dordrecht, Netherlands), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- The Hedgehog/GLI signaling pathway activates transcription of Slug (Snail2) in melanoma cells.Oncology reports · 2023Article
- LINC01224 facilitates the proliferation and inhibits the radiosensitivity of melanoma cells through the miR-193a-5p/NR1D2 axis.The Kaohsiung journal of medical sciences · 2022Article
- HDAC1 promotes the migration of human myeloma cells via regulation of the lncRNA/Slug axis.International journal of molecular medicine · 2022Article
- The long non-coding RNA HOTAIRM1 promotes tumor aggressiveness and radiotherapy resistance in glioblastoma.Cell death & disease · 2021Article
- HOXC10 promotes growth and migration of melanoma by regulating Slug to activate the YAP/TAZ signaling pathway.Discover oncology · 2021Article
- CXCR4 uses STAT3-mediated slug expression to maintain radioresistance of non-small cell lung cancer cells: emerges as a potential prognostic biomarker for lung cancer.Cell death & disease · 2021Article
- Molecular regulation of Snai2 in development and disease.Journal of cell science · 2019Review
- Effect of modulation of epithelial-mesenchymal transition regulators Snail1 and Snail2 on cancer cell radiosensitivity by targeting of the cell cycle, cell apoptosis and cell migration/invasion.Oncology letters · 2019Review
- Slug inhibition increases radiosensitivity of nasopharyngeal carcinoma cell line C666-1.Experimental and therapeutic medicine · 2018Article
- Preclinical evidence of multiple mechanisms underlying trastuzumab resistance in gastric cancer.Oncotarget · 2016Article
- Manipulation of tumor metabolism for therapeutic approaches: ovarian cancer-derived cell lines as a model system.Cellular oncology (Dordrecht, Netherlands) · 2015Article
- Fatal autoimmunity results from the conditional deletion of Snai2 and Snai3.Cellular immunology · 2015Article
- CTC1 increases the radioresistance of human melanoma cells by inhibiting telomere shortening and apoptosis.International journal of molecular medicine · 2014Article
- New biomarkers to predict the evolution of in situ breast cancers.BioMed research international · 2014Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMelanoma radioresistance has been attributed to the presence of tumor cells with highly efficient DNA damage repair mechanisms. We examined the expression of genes involved in DNA damage repair and DNA damage sensing, and assessed their modulation by SLUG silencing, which is potentially capable of increasing radiosensitivity.
methodsTwo melanoma cell lines (M14 and M79) were used to evaluate in vitro radiation-induced cytotoxicity before and after SLUG silencing. mRNA expression levels of BRCA1, ERCC1, DNA-PK, PARP, MGMT, ATM and TGM2 were determined by real-time RT-PCR, and protein expression levels of SLUG, caspase 3, p21, PUMA and pMAPK by Western blotting.
resultsThe cytotoxic effect of radiation was high in M14 and low in M79 cells. SLUG silencing increased the interference of radiation on cell cycle distribution and cell killing by 60 % and 80 % in M79 cells after a 2.4 Gy and 5 Gy radiation dose, respectively. It also led to a significant inhibition of expression of genes involved in DNA damage repair and DNA damage sensing in all cell lines maintained after radiation. An almost total inhibition was observed for TGM2, which is expressed at a high basal level in the most radioresistant cell line (M79). Protein expression of PUMA was induced by radiation and was enhanced after SLUG silencing.
conclusionsOur results reveal a pivotal role of SLUG in regulating a cellular network involved in the response to DNA damage, and highlight the importance of TGM2 in radiosensitivity modulation. SLUG silencing appears to increase radiation sensitivity of the melanoma cells tested.
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