ArticleNature communications2012
Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 68 citations in OpenAlex.
- Trial
- Trial
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- Generation of a mouse SWATH-MS spectral library to quantify 10148 proteins involved in cell reprogramming.Scientific data · 2021Article
- Proteomic Applications in Antimicrobial Resistance and Clinical Microbiology Studies.Infection and drug resistance · 2020Review
- A quantitative Streptococcus pyogenes-human protein-protein interaction map reveals localization of opsonizing antibodies.Nature communications · 2019Article
- Purple: A Computational Workflow for Strategic Selection of Peptides for Viral Diagnostics Using MS-Based Targeted Proteomics.Viruses · 2019Article
- Greedy de novo motif discovery to construct motif repositories for bacterial proteomes.BMC bioinformatics · 2019Article
- Rapid determination of quaternary protein structures in complex biological samples.Nature communications · 2019Article
- Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.Journal of proteomics · 2018Review
- Data-independent acquisition-based SWATH-MS for quantitative proteomics: a tutorial.Molecular systems biology · 2018Review
- Development of Large-scale Cross-linking Mass Spectrometry.Molecular & cellular proteomics : MCP · 2018Article
- Statistical control of peptide and protein error rates in large-scale targeted data-independent acquisition analyses.Nature methods · 2017Article
- Targeted Proteomics and Absolute Protein Quantification for the Construction of a Stoichiometric Host-Pathogen Surface Density Model.Molecular & cellular proteomics : MCP · 2017Article
- Review
- Towards comprehensive and quantitative proteomics for diagnosis and therapy of human disease.Proteomics · 2017Review
- Lactic acid bacterial symbionts in honeybees - an unknown key to honey's antimicrobial and therapeutic activities.International wound journal · 2016Article
- Saliva-Induced Clotting Captures Streptococci: Novel Roles for Coagulation and Fibrinolysis in Host Defense and Immune Evasion.Infection and immunity · 2016Article
- Article
- Dinosaur: A Refined Open-Source Peptide MS Feature Detector.Journal of proteome research · 2016Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
Selected reaction monitoring mass spectrometry (SRM-MS) is a targeted proteomics technology used to identify and quantify proteins with high sensitivity, specificity and high reproducibility. Execution of SRM-MS relies on protein-specific SRM assays, a set of experimental parameters that requires considerable effort to develop. Here we present a proteome-wide SRM assay repository for the gram-positive human pathogen group A Streptococcus. Using a multi-layered approach we generated SRM assays for 10,412 distinct group A Streptococcus peptides followed by extensive testing of the selected reaction monitoring assays in >200 different group A Streptococcus protein pools. Based on the number of SRM assay observations we created a rule-based selected reaction monitoring assay-scoring model to select the most suitable assays per protein for a given cellular compartment and bacterial state. The resource described here represents an important tool for deciphering the group A Streptococcus proteome using selected reaction monitoring and we anticipate that concepts described here can be extended to other pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.