ArticleNature communications2012
A visible dominant marker for insect transgenesis.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Ectopic Wnt6 expression induces twin-spot markings in the epidermis of the silkworm Bombyx mori - Potential molecular target to discover evolutionary mechanisms of adaptive body coloration in insects.The FEBS journal · 2026Article
- Similar Genetic Routes Are Independently Targeted for Mimetic Color Convergence in Bumble Bees.Molecular biology and evolution · 2025Article
- Production of Transgenic Silkworm Using Anti-Serum Against Diapause Hormone in Diapause Strains of Silkworm,International journal of molecular sciences · 2025Article
- Egg Microinjection for the Ladybird BeetleBio-protocol · 2024Article
- Egg Cooling After Oviposition Extends the Permissive Period for Microinjection-Mediated Genome Modification inInternational journal of molecular sciences · 2024Article
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- A toolbox of engineered mosquito lines to study salivary gland biology and malaria transmission.PLoS pathogens · 2022Article
- A deterministic genotyping workflow reduces waste of transgenic individuals by two-thirds.Scientific reports · 2021Article
- Comparative transcriptomics of albino and warningly-coloured caterpillars.Ecology and evolution · 2021Article
- Genetic Manipulation of Ticks: A Paradigm Shift in Tick and Tick-Borne Diseases Research.Frontiers in cellular and infection microbiology · 2021Review
- Article
- An essential role of acetyl coenzyme A in the catalytic cycle of insect arylalkylamine N-acetyltransferase.Communications biology · 2020Article
- Three Melanin Pathway Genes,International journal of molecular sciences · 2019Article
- Article
- Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein.Genetics · 2017Article
- Effects of starvation on the carbohydrate metabolism inBiology open · 2017Article
- Acetyl group coordinated progression through the catalytic cycle of an arylalkylamine N-acetyltransferase.PloS one · 2017Article
- Critical Analysis of the Melanogenic Pathway in Insects and Higher Animals.International journal of molecular sciences · 2016Review
- Metabolomics Analysis of the Larval Head of the Silkworm, Bombyx mori.International journal of molecular sciences · 2016Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.