ArticlePlant methods2012
Inflorescence stem grafting made easy in Arabidopsis.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The polar-localized borate exporter BOR1 facilitates boron transport in tapetal cells to the developing pollen grains.Plant physiology · 2025Article
- Advances in understanding the graft healing mechanism: a review of factors and regulatory pathways.Horticulture research · 2024Article
- A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing.Nature plants · 2024Article
- Article
- Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study.Plant methods · 2022Article
- Use of Micrografting to Study the Role Played by Peptide Signals in ABA Biosynthesis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Peptide Hormone Receptor CEPR1 Functions in the Reproductive Tissue to Control Seed Size and Yield.Plant physiology · 2020Article
- Cut and paste: temperature-enhanced cotyledon micrografting forPlant methods · 2020Article
- Insights Into Plant Surgery: An Overview of the Multiple Grafting Techniques forFrontiers in plant science · 2020Review
- Methods for graftingPlant methods · 2019Article
- Plant grafting: insights into tissue regeneration.Regeneration (Oxford, England) · 2017Review
- tRNA-Related Sequences Trigger Systemic mRNA Transport in Plants.The Plant cell · 2016Article
- Arabidopsis MSH1 mutation alters the epigenome and produces heritable changes in plant growth.Nature communications · 2015Article
- A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages.Plant methods · 2015Article
- Article
- An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings.Plant methods · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundPlant grafting techniques have deepened our understanding of the signals facilitating communication between the root and shoot, as well as between shoot and reproductive organs. Transmissible signalling molecules can include hormones, peptides, proteins and metabolites: some of which travel long distances to communicate stress, nutrient status, disease and developmental events. While hypocotyl micrografting techniques have been successfully established for Arabidopsis to explore root to shoot communications, inflorescence grafting in Arabidopsis has not been exploited to the same extent. Two different strategies (horizontal and wedge-style inflorescence grafting) have been developed to explore long distance signalling between the shoot and reproductive organs. We developed a robust wedge-cleft grafting method, with success rates greater than 87%, by developing better tissue contact between the stems from the inflorescence scion and rootstock. We describe how to perform a successful inflorescence stem graft that allows for reproducible translocation experiments into the physiological, developmental and molecular aspects of long distance signalling events that promote reproduction.
resultsWedge grafts of the Arabidopsis inflorescence stem were supported with silicone tubing and further sealed with parafilm to maintain the vascular flow of nutrients to the shoot and reproductive tissues. Nearly all (87%) grafted plants formed a strong union between the scion and rootstock. The success of grafting was scored using an inflorescence growth assay based upon the growth of primary stem. Repeated pruning produced new cauline tissues, healthy flowers and reproductive siliques, which indicates a healthy flow of nutrients from the rootstock. Removal of the silicone tubing showed a tightly fused wedge graft junction with callus proliferation. Histological staining of sections through the graft junction demonstrated the differentiation of newly formed vascular connections, parenchyma tissue and lignin accumulation, supporting the presumed success of the graft union between two sections of the primary inflorescence stem.
conclusionsWe describe a simple and reliable method for grafting sections of an Arabidopsis inflorescence stem. This step-by-step protocol facilitates laboratories without grafting experience to further explore the molecular and chemical signalling which coordinates communications between the shoot and reproductive tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.