ArticleFrontiers in neurology2012
Comprehensive Method for Culturing Embryonic Dorsal Root Ganglion Neurons for Seahorse Extracellular Flux XF24 Analysis.
Article in Frontiers in neurology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Low-Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation.Journal of neurochemistry · 2025Article
- Isolation of Intact Mitochondria FromBio-protocol · 2025Article
- Developing an approach for evaluating the cardiotoxic potential of botanicals.Frontiers in toxicology · 2025Review
- Recent Approaches to Determine Static and Dynamic Redox State-Related Parameters.Antioxidants (Basel, Switzerland) · 2022Review
- Gypenoside XVII protects against myocardial ischemia and reperfusion injury by inhibiting ER stress-induced mitochondrial injury.Journal of ginseng research · 2021Article
- Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test.STAR protocols · 2021Article
- Differential effect of amyloid beta peptides on mitochondrial axonal trafficking depends on their state of aggregation and binding to the plasma membrane.Neurobiology of disease · 2018Article
- A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma.The Journal of biological chemistry · 2016Article
- The cell and molecular biology of neurodegenerative diseases: an overview.Frontiers in neurology · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Changes in mitochondrial dynamics and function contribute to progression of multiple neurodegenerative diseases including peripheral neuropathies. The Seahorse Extracellular Flux XF24 analyzer provides a comprehensive assessment of the relative state of glycolytic and aerobic metabolism in live cells making this method instrumental in assessing mitochondrial function. One of the most important steps in the analysis of mitochondrial respiration using the Seahorse XF24 analyzer is plating a uniform monolayer of firmly attached cells. However, culturing of primary dorsal root ganglion (DRG) neurons is associated with multiple challenges, including their propensity to form clumps and detach from the culture plate. This could significantly interfere with proper analysis and interpretation of data. We have tested multiple cell culture parameters including coating substrates, culture medium, XF24 microplate plastics, and plating techniques in order to optimize plating conditions. Here we describe a highly reproducible method to obtain neuron-enriched monolayers of securely attached dissociated primary embryonic (E15) rat DRG neurons suitable for analysis with the Seahorse XF24 platform.
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