ArticleNucleic acids research2013
Magnetic tweezers measurements of the nanomechanical stability of DNA against denaturation at various conditions of pH and ionic strength.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 46 citations in OpenAlex.
- The effects of DMSO on DNA conformations and mechanics.Biophysical journal · 2025Article
- DNA binding to small anionic ligands: the case of Orange G dye.European biophysics journal : EBJ · 2025Article
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- The incipient denaturation mechanism of DNA.RSC advances · 2022Article
- Nanomechanics of negatively supercoiled diaminopurine-substituted DNA.Nucleic acids research · 2021Article
- Nanomechanics of G-quadruplexes within the promoter of the KIT oncogene.Nucleic acids research · 2021Article
- A Primer on the oxDNA Model of DNA: When to Use it, How to Simulate it and How to Interpret the Results.Frontiers in molecular biosciences · 2021Review
- Nanomechanics of Diaminopurine-Substituted DNA.Biophysical journal · 2019Article
- The temperature dependence of the helical twist of DNA.Nucleic acids research · 2018Article
- Transcription-induced supercoiling as the driving force of chromatin loop extrusion during formation of TADs in interphase chromosomes.Nucleic acids research · 2018Article
- Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.Nucleic acids research · 2017Article
- Probing the salt dependence of the torsional stiffness of DNA by multiplexed magnetic torque tweezers.Nucleic acids research · 2017Article
- Annealing helicase HARP closes RPA-stabilized DNA bubbles non-processively.Nucleic acids research · 2017Article
- Platinum-Based Drugs and DNA Interactions Studied by Single-Molecule and Bulk Measurements.Biophysical journal · 2016Article
- Plectoneme tip bubbles: coupled denaturation and writhing in supercoiled DNA.Scientific reports · 2015Article
- Article
- Article
- Optical characterization of oligonucleotide DNA influenced by magnetic fields.Molecules (Basel, Switzerland) · 2013Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considering the classical dependence of DNA melting temperature on pH and salt concentration. In the present work, we report a single-molecule study of the stability of DNA against denaturation when subjected to changes in solvent. We investigated the appearance of DNA instability under specific external applied force and imposed twist values, which was revealed by an increase in the temporal fluctuations in the DNA extension. These fluctuations occur in the presence of a continuous interval of equilibrium states, ranging from a plectonemic state to a state characterized by denaturation bubbles. In particular, we observe the fluctuations only around a characteristic force value. Moreover, this characteristic force is demonstrated to be notably sensitive to variations in the pH and ionic strength. Finally, an extension of a theoretical model of plectoneme formation is used to estimate the average denaturation energy, which is found to be linearly correlated to the melting temperature of the DNA double strands.
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