Evidence map›Paper›PMID 23247526›Full record

ArticleEnvironmental science and pollution research international2013

The quantitative real-time PCR applications in the monitoring of marine harmful algal bloom (HAB) species.

Antonella Penna, Penna Antonella, Luca Galluzzi, Galluzzi Luca

Erratum issuedOpen access · hybridAbstract read
In one paragraph

Article in Environmental science and pollution research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
6.3field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 106 citations in OpenAlex.

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4 · The record

Corrections and comments

  • Erratum issued
    Antonella, Penna [corrected to Penna, Antonella]; Luca, Galluzzi [corrected to Galluzzi, Luca]
5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Antonella PennaDepartment of Biomolecular Sciences, University of Urbino, Viale Trieste 296, Pesaro, Italy, antonella.penna@uniurb.it.
Penna AntonellaDepartment of Biomolecular Sciences, University of Urbino, Viale Trieste 296, Pesaro, Italy, antonella.penna@uniurb.it.
Luca Galluzzi
Galluzzi Luca
University of Urbino · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the last decade, various molecular methods (e.g., fluorescent hybridization assay, sandwich hybridization assay, automatized biosensor detection, real-time PCR assay) have been developed and implemented for accurate and specific identification and estimation of marine toxic microalgal species. This review focuses on the recent quantitative real-time PCR (qrt-PCR) technology developed for the control and monitoring of the most important taxonomic phytoplankton groups producing biotoxins with relevant negative impact on human health, the marine environment, and related economic activities. The high specificity and sensitivity of the qrt-PCR methods determined by the adequate choice of the genomic target gene, nucleic acid purification protocol, quantification through the standard curve, and type of chemical detection method make them highly efficient and therefore applicable to harmful algal bloom phenomena. Recent development of qrt-PCR-based assays using the target gene of toxins, such as saxitoxin compounds, has allowed more precise quantification of toxigenic species (i.e., Alexandrium catenella) abundance. These studies focus only on toxin-producing species in the marine environment. Therefore, qrt-PCR technology seems to offer the advantages of understanding the ecology of harmful algal bloom species and facilitating the management of their outbreaks.

Indexed as

Harmful Algal BloomReal-Time Polymerase Chain ReactionDinoflagellidaEnvironmental MonitoringHumansMarine ToxinsNucleic Acid HybridizationPhytoplanktonSaxitoxinSeawaterSensitivity and SpecificityWater PollutionMarine ToxinsSaxitoxin

Identifiers

PMID23247526
PMCPMC3782655
OpenAlexW1994848574

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.