ArticleAnaerobe2013
A systematic evaluation of methods to optimize culture-based recovery of Clostridium difficile from stool specimens.
Article in Anaerobe, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02046512 (Randomized Controlled Trial of Lactobacillus Rhamnosus GG to Promote Intestinal Health), which is not on this map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Randomized Controlled Trial of Lactobacillus Rhamnosus GG to Promote Intestinal Health
Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
- Randomized Controlled Trial of Oral Vancomycin Treatment in Clostridioides difficile-Colonized Patients.mSphere · 2021Trial
- Randomized Controlled Trial to Determine the Impact of Probiotic Administration on Colonization With Multidrug-Resistant Organisms in Critically Ill Patients.Infection control and hospital epidemiology · 2015Trial
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- Comparative evaluation of the STANDARD M10 and XpertJournal of clinical microbiology · 2024Article
- Comparison ofJournal of clinical microbiology · 2024Article
- Diagnostic Guidance for C. difficile Infections.Advances in experimental medicine and biology · 2024Article
- Clostridioides (Clostridium) difficile: A silent nosocomial pathogen.Saudi medical journal · 2023Review
- Overview of current detection methods and microRNA potential inWorld journal of gastroenterology · 2023Review
- Laboratory diagnosis of Clostridioides difficile infection in symptomatic patients: what can we do better?Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Probiotic Bacteria from Human Milk Can Alleviate Oral Bovine Casein Sensitization in Juvenile Wistar Rats.Microorganisms · 2023Article
- Molecular epidemiology ofAntimicrobial stewardship & healthcare epidemiology : ASHE · 2023Article
- Alternative Causes of Infectious Diarrhea in Patients with Negative Tests for Clostridoides Difficile.The journal of applied laboratory medicine · 2022Article
- Evaluation of an immunochromatographic test for the detection of glutamate dehydrogenase for the diagnosis of Clostridioides (Clostridium) difficile infection in dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Evaluation of glutamate dehydrogenase (GDH) and toxin A/B rapid tests for Clostridioides (prev. Clostridium) difficile diagnosis in a university hospital in Minas Gerais, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Laboratory Tests for the Diagnosis ofClinics in colon and rectal surgery · 2020Review
- Impact of an electronic hard-stop clinical decision support tool to limit repeatInfection control and hospital epidemiology · 2019Article
- Spatiotemporal dynamics of multidrug resistant bacteria on intensive care unit surfaces.Nature communications · 2019Article
- Metabolomic networks connect host-microbiome processes to human Clostridioides difficile infections.The Journal of clinical investigation · 2019Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
With the increasing prevalence of Clostridium difficile infection among hospitalized patients, a clear understanding of C. difficile epidemiology is needed to evaluate current prevention policies, and to create new and effective policies. To determine the epidemiology of C. difficile, the most sensitive methods for detection of C. difficile are required. The purpose of this study was to systematically assess multiple methods to determine the most sensitive method to recover C. difficile from stool and rectal swabs. Stool samples from healthy asymptomatic individuals that were collected and confirmed to be culture negative for C. difficile were spiked with known concentrations of C. difficile ranging from 10(4) CFU of C. difficile per ml of stool to 10 CFU per ml. Two sets of experiments (A and B) were performed and each involved multiple combinations of untreated and treated stool/rectal swab specimens and selective and non-selective broth and agar. Overall, recovery of C. difficile was increased with the use of an initial broth enrichment followed by plating to solid medium. The most sensitive method of C. difficile detection for both stools and swab specimens was heat shock prior to inoculation of cycloserine-cefoxitin mannitol broth with taurocholate lysozyme cysteine (CCMB, Anaerobe Systems, Morgan Hill, CA) followed by isolation from pre-reduced TSA II with 5% sheep blood (BAP, BBL BD and Co., Sparks, MD). Identifying the most sensitive method of recovery will allow for further study of asymptomatic C. difficile carriers and their role in the epidemiology of C. difficile.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.