ArticleBMC microbiology2012
Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
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- Class 1 integrons and plasmid-mediated multiple resistance genes of theGut pathogens · 2017Article
- Evaluation of the microbiological quality of reclaimed water produced from a lagooning system.Environmental science and pollution research international · 2016Article
- The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofBioMed research international · 2016Article
- Higher water temperature and incubation under aerobic and microaerobic conditions increase the recovery and diversity of Arcobacter spp. from shellfish.Applied and environmental microbiology · 2014Article
- Performance of five molecular methods for monitoring Arcobacter spp.BMC microbiology · 2013Article
- Adherence to and invasion of human intestinal cells by Arcobacter species and their virulence genotypes.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundArcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease (MseI). The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter.
resultsDigestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and/or BfaI generated species-specific RFLP patterns.
conclusions16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses.
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