ArticleMalaria journal2012
A rapid and scalable density gradient purification method for Plasmodium sporozoites.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- Effective Antimalarial Chemoprevention in Childhood Enhances the Quality of CD4+ T Cells and Limits Their Production of Immunoregulatory Interleukin 10.The Journal of infectious diseases · 2016Trial
- A pleckstrin homology domain protein is involved in crystalloid formation inFrontiers in cellular and infection microbiology · 2026Article
- Protocol for laboratory rearing and infection tracking of Rhodnius prolixus using 3D-printable designs.STAR protocols · 2025Article
- A 3D culture model facilitates mass production of in vitro Plasmodium falciparum haemolymph-like sporozoites.Malaria journal · 2025Article
- Androgens inhibit protective CD8Nature communications · 2025Article
- Parasite and vector circadian clocks mediate efficient malaria transmission.Nature microbiology · 2025Article
- Protective antibodies target cryptic epitope unmasked by cleavage of malaria sporozoite protein.Science (New York, N.Y.) · 2025Article
- Identification of a novel rhoptry protein expressed predominantly inFrontiers in cellular and infection microbiology · 2025Article
- Identifying Plasmodium P36 and P52 antigens for coadministration with circumsporozoite protein to enhance vaccine efficacy.NPJ vaccines · 2024Article
- IdentifyingResearch square · 2024Article
- Article
- Plasmodium GPI-anchored micronemal antigen is essential for parasite transmission through the mosquito host.Molecular microbiology · 2024Article
- Ultra-low volume intradermal administration of radiation-attenuated sporozoites with the glycolipid adjuvant 7DW8-5 completely protects mice against malaria.Scientific reports · 2024Article
- Accelerated prime-and-trap vaccine regimen in mice using repRNA-based CSP malaria vaccine.NPJ vaccines · 2024Article
- More time to kill: A longer liver stage increases T cell-mediated protection against pre-erythrocytic malaria.iScience · 2023Article
- Chemo-proteomics in antimalarial target identification and engagement.Medicinal research reviews · 2023Review
- Optimization of Plasmodium vivax infection of colonized Amazonian Anopheles darlingi.Scientific reports · 2023Article
- Rhoptry neck protein 4 plays important roles duringmSphere · 2023Article
- Plasmodium falciparum Gametes and Sporozoites Hijack Plasmin and Factor H To Evade Host Complement Killing.Microbiology spectrum · 2023Article
- Antibody-dependent immune responses elicited by blood stage-malaria infection contribute to protective immunity to the pre-erythrocytic stages.Current research in immunology · 2023Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors.
Funding
Abstract
backgroundMalaria remains a major human health problem, with no licensed vaccine currently available. Malaria infections initiate when infectious Plasmodium sporozoites are transmitted by Anopheline mosquitoes during their blood meal. Investigations of the malaria sporozoite are, therefore, of clear medical importance. However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes.
methodsHere is described a discontinuous density gradient purification method for Plasmodium sporozoites that maintains parasite infectivity in vitro and in vivo and greatly reduces mosquito and microbial contaminants.
resultsThis method provides clear advantages over previous approaches: it is rapid, requires no serum components, and can be scaled to purify >107 sporozoites with minimal operator involvement. Moreover, it can be effectively applied to both human (Plasmodium falciparum, Plasmodium vivax) and rodent (Plasmodium yoelii) infective species with excellent recovery rates.
conclusionsThis novel method effectively purifies viable malaria sporozoites by greatly reducing contaminating mosquito debris and microbial burdens associated with parasite isolation. Large-scale preparations of purified sporozoites will allow for enhanced in vitro infections, proteomics, and biochemical characterizations. In conjunction with aseptic mosquito rearing techniques, this purification technique will also support production of live attenuated sporozoites for vaccination.
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