ArticleNano letters2013
Probing enzymatic activity inside living cells using a nanowire-cell "sandwich" assay.
Article in Nano letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Just a little prick: careful cell contacts enabled by ceramic nanostraws.Pflugers Archiv : European journal of physiology · 2026Article
- Nanoinjection: A Platform for Innovation in Ex Vivo Cell Engineering.Accounts of chemical research · 2024Article
- Controlled degradation of polycaprolactone-based micropillar arrays.Biomaterials science · 2023Article
- Microfluidic mechanoporation for cellular delivery and analysis.Materials today. Bio · 2022Review
- Fluorescent Biosensors Based on Silicon Nanowires.Nanomaterials (Basel, Switzerland) · 2021Review
- Tutorial: using nanoneedles for intracellular delivery.Nature protocols · 2021Review
- Analysis of Actin and Focal Adhesion Organisation in U2OS Cells on Polymer Nanostructures.Nanoscale research letters · 2021Article
- Nanowire Assisted Mechanotyping of Cellular Metastatic Potential.Advanced functional materials · 2021Article
- Nanoneedle-Based Materials for Intracellular Studies.Advances in experimental medicine and biology · 2021Article
- Optical Transduction for Vertical Nanowire Resonators.Nano letters · 2020Article
- The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling.Frontiers in chemistry · 2020Review
- Nanostructured Materials for Intracellular Cargo Delivery.Accounts of chemical research · 2019Review
- Functionalized Spiky Particles for Intracellular Biomolecular Delivery.ACS central science · 2019Article
- Uptake of nanowires by human lung adenocarcinoma cells.PloS one · 2019Article
- Biofunctionalized 3D Nanopillar Arrays Fostering Cell Guidance and Promoting Synapse Stability and Neuronal Activity in Networks.ACS applied materials & interfaces · 2018Article
- Spatially Correlated, Single Nanomaterial-Level Structural and Optical Profiling of Cu-Doped ZnO Nanorods Synthesized via Multifunctional Silicides.Nanomaterials (Basel, Switzerland) · 2018Article
- Roadmap on semiconductor-cell biointerfaces.Physical biology · 2018Review
- Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Micro- and Nanoscale Technologies for Delivery into Adherent Cells.Trends in biotechnology · 2016Review
- From immobilized cells to motile cells on a bed-of-nails: effects of vertical nanowire array density on cell behaviour.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs' ability to penetrate cellular membranes without affecting cells' viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay's potential in uncovering the physiological roles of various enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.