ArticleBiomedical optics express2012
High-resolution 3D optical microscopy inside the beating zebrafish heart using prospective optical gating.
Article in Biomedical optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- PAAQ: Paired Alternating AcQuisitions for virtual high frame rate multichannel cardiac fluorescence microscopy.Biological imaging · 2023Article
- Haemodynamic dependence of mechano-genetic evolution of the cardiovascular system in Japanese medaka.Journal of the Royal Society, Interface · 2021Article
- Real-time multi-angle projection imaging of biological dynamics.Nature methods · 2021Article
- High-contrast, synchronous volumetric imaging with selective volume illumination microscopy.Communications biology · 2020Article
- Automated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions.Scientific reports · 2020Article
- Live Imaging of Heart Injury in Larval Zebrafish Reveals a Multi-Stage Model of Neutrophil and Macrophage Migration.Frontiers in cell and developmental biology · 2020Article
- Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart.Nature communications · 2019Article
- Second harmonic generation microscopy of early embryonic mouse hearts.Biomedical optics express · 2019Article
- Spatial and temporal variations in hemodynamic forces initiate cardiac trabeculation.JCI insight · 2018Article
- 3D + time blood flow mapping using SPIM-microPIV in the developing zebrafish heart.Biomedical optics express · 2018Article
- Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model.Toxicological sciences : an official journal of the Society of Toxicology · 2017Article
- Compact plane illumination plugin device to enable light sheet fluorescence imaging of multi-cellular organisms on an inverted wide-field microscope.Biomedical optics express · 2016Article
- Intravital imaging of cardiac function at the single-cell level.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Optically gated beating-heart imaging.Frontiers in physiology · 2014Review
- Moving domain computational fluid dynamics to interface with an embryonic model of cardiac morphogenesis.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3D fluorescence imaging is a fundamental tool in the study of functional and developmental biology, but effective imaging is particularly difficult in moving structures such as the beating heart. We have developed a non-invasive real-time optical gating system that is able to exploit the periodic nature of the motion to acquire high resolution 3D images of the normally-beating zebrafish heart without any unnecessary exposure of the sample to harmful excitation light. In order for the image stack to be artefact-free, it is essential to use a synchronization source that is invariant as the sample is scanned in 3D. We therefore describe a scheme whereby fluorescence image slices are scanned through the sample while a brightfield camera sharing the same objective lens is maintained at a fixed focus, with correction of sample drift also included. This enables us to maintain, throughout an extended 3D volume, the same standard of synchronization we have previously demonstrated in and near a single 2D plane. Thus we are able image the complete beating zebrafish heart exactly as if the heart had been artificially stopped, but sidestepping this undesirable interference with the heart and instead allowing the heart to beat as normal.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.