ArticleAnnals of botany2013
A triallelic genetic male sterility locus in Brassica napus: an integrative strategy for its physical mapping and possible local chromosome evolution around it.
Article in Annals of botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 19 citations in OpenAlex.
- Development of the PARMS Marker of the Dominant Genic Male Sterility (DGMS) Line and Its Utilization in Rapeseed (Plants (Basel, Switzerland) · 2022Article
- Evolution of the Brassicaceae-specific MS5-Like family and neofunctionalization of the novel MALE STERILITY 5 gene essential for male fertility in Brassica napus.The New phytologist · 2021Article
- Cytological and genetic characterisation of dominant GMS line Shaan-GMS in Brassica napus L.Journal of applied genetics · 2020Article
- Rolling Circle Amplification (RCA)-Mediated Genome-Wide ihpRNAi Mutant Library Construction inInternational journal of molecular sciences · 2020Article
- Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene inG3 (Bethesda, Md.) · 2020Article
- Mapping of the male sterile mutant gene ftms in Brassica rapa L. ssp. pekinensis via BSR-Seq combined with whole-genome resequencing.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- MS5 Mediates Early Meiotic Progression and Its Natural Variants May Have Applications for Hybrid Production in Brassica napus.The Plant cell · 2016Article
- Identification and Analysis ofFrontiers in plant science · 2016Article
- Map-based cloning reveals the complex organization of the BnRf locus and leads to the identification of BnRf(b), a male sterility gene, in Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- High-Density SNP Map Construction and QTL Identification for the Apetalous Character in Brassica napus L.Frontiers in plant science · 2015Article
- A complex recombination pattern in the genome of allotetraploid Brassica napus as revealed by a high-density genetic map.PloS one · 2014Article
- Targeted sequence capture provides insight into genome structure and genetics of male sterility in a gynodioecious diploid strawberry, Fragaria vesca ssp. bracteata (Rosaceae).G3 (Bethesda, Md.) · 2013Article
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6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsSpontaneous male sterility is an advantageous trait for both constructing efficient pollination control systems and for understanding the developmental process of the male reproductive unit in many crops. A triallelic genetic male-sterile locus (BnMs5) has been identified in Brassica napus; however, its complicated genome structure has greatly hampered the isolation of this locus. The aim of this study was to physically map BnMs5 through an integrated map-based cloning strategy and analyse the local chromosomal evolution around BnMs5.
methodsA large F(2) population was used to integrate the existing genetic maps around BnMs5. A map-based cloning strategy in combination with comparative mapping among B. napus, Arabidopsis, Brassica rapa and Brassica oleracea was employed to facilitate the identification of a target bacterial artificial chromosome (BAC) clone covering the BnMs5 locus. The genomic sequences from the Brassica species were analysed to reveal the regional chromosomal evolution around BnMs5. KEY
resultsBnMs5 was finally delimited to a 0·3-cM genetic fragment from an integrated local genetic map, and was anchored on the B. napus A8 chromosome. Screening of a B. napus BAC clone library and identification of the positive clones validated that JBnB034L06 was the target BAC clone. The closest flanking markers restrict BnMs5 to a 21-kb region on JBnB034L06 containing six predicted functional genes. Good collinearity relationship around BnMs5 between several Brassica species was observed, while violent chromosomal evolutionary events including insertions/deletions, duplications and single nucleotide mutations were also found to have extensively occurred during their divergence.
conclusionsThis work represents major progress towards the molecular cloning of BnMs5, as well as presenting a powerful, integrative method to mapping loci in plants with complex genomic architecture, such as the amphidiploid B. napus.
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