ArticleMolecular biology of the cell2013
Yeast G-proteins mediate directional sensing and polarization behaviors in response to changes in pheromone gradient direction.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 21 citations in OpenAlex.
- Information bounds on the accuracy of cell polarization.PloS one · 2025Article
- An arbitrary Lagrangian Eulerian smoothed particle hydrodynamics (ALE-SPH) method with a boundary volume fraction formulation for fluid-structure interaction.Engineering analysis with boundary elements · 2021Article
- Exploratory polarization facilitates mating partner selection inMolecular biology of the cell · 2021Article
- Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.Molecular biology of the cell · 2020Article
- Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.The Journal of biological chemistry · 2019Article
- Quantitative analysis of the yeast pheromone pathway.Yeast (Chichester, England) · 2019Article
- A Model for Direction Sensing in Dictyostelium discoideum: Ras Activity and Symmetry Breaking Driven by a Gβγ-Mediated, Gα2-Ric8 -- Dependent Signal Transduction Network.PLoS computational biology · 2016Article
- A Predictive Model for Yeast Cell Polarization in Pheromone Gradients.PLoS computational biology · 2016Article
- Alternative cell polarity behaviours arise from changes in G-protein spatial dynamics.IET systems biology · 2015Article
- RGS proteins and septins cooperate to promote chemotropism by regulating polar cap mobility.Current biology : CB · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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Abstract
Yeast cells polarize by projecting up mating pheromone gradients, a classic cell polarity behavior. However, these chemical gradients may shift direction. We examine how yeast cells sense and respond to a 180(o) switch in the direction of microfluidically generated pheromone gradients. We identify two behaviors: at low concentrations of α-factor, the initial projection grows by bending, whereas at high concentrations, cells form a second projection toward the new source. Mutations that increase heterotrimeric G-protein activity expand the bending-growth morphology to high concentrations; mutations that increase Cdc42 activity result in second projections at low concentrations. Gradient-sensing projection bending requires interaction between Gβγ and Cdc24, whereas gradient-nonsensing projection extension is stimulated by Bem1 and hyperactivated Cdc42. Of interest, a mutation in Gα affects both bending and extension. Finally, we find a genetic perturbation that exhibits both behaviors. Overexpression of the formin Bni1, a component of the polarisome, makes both bending-growth projections and second projections at low and high α-factor concentrations, suggesting a role for Bni1 downstream of the heterotrimeric G-protein and Cdc42 during gradient sensing and response. Thus we demonstrate that G-proteins modulate in a ligand-dependent manner two fundamental cell-polarity behaviors in response to gradient directional change.
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