ArticlePlant molecular biology2013
Enhanced Agrobacterium-mediated transformation efficiencies in monocot cells is associated with attenuated defense responses.
Article in Plant molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 80 citations in OpenAlex.
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- The LpHsfA2-molecular module confers thermotolerance via fine tuning of its transcription in perennial ryegrass (Lolium perenne L.).Journal of integrative plant biology · 2024Article
- MicroRNA164 Affects Plant Responses to UV Radiation in Perennial Ryegrass.Plants (Basel, Switzerland) · 2024Article
- Antivirulence effects of cell-free culture supernatant of endophytic bacteria against grapevine crown gall agent, Agrobacterium tumefaciens, and induction of defense responses in plantlets via intact bacterial cells.BMC plant biology · 2024Article
- An EfficientPlants (Basel, Switzerland) · 2023Article
- Callus Induction from Diverse Explants and Genotypes Enables Robust Transformation of Perennial Ryegrass (Plants (Basel, Switzerland) · 2022Article
- The NAC factor LpNAL delays leaf senescence by repressing two chlorophyll catabolic genes in perennial ryegrass.Plant physiology · 2022Article
- Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation.Nature communications · 2022Article
- Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- Heterologous expression of a chimeric gene, OsDST-SRDX, enhanced salt tolerance of transgenic switchgrass (Panicum virgatum L.).Plant cell reports · 2020Article
- A New Plant Expression System for Producing Pharmaceutical Proteins.Molecular biotechnology · 2020Article
- An improved protocol for efficient transformation and regeneration of Setaria italica.Plant cell reports · 2020Article
- A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals.Plant cell, tissue and organ culture · 2020Article
- Transgenic creeping bentgrass plants expressing a Picea wilsonii dehydrin gene (PicW) demonstrate improved freezing tolerance.Molecular biology reports · 2018Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant defense responses can lead to altered metabolism and even cell death at the sites of Agrobacterium infection, and thus lower transformation frequencies. In this report, we demonstrate that the utilization of culture conditions associated with an attenuation of defense responses in monocot plant cells led to highly improved Agrobacterium-mediated transformation efficiencies in perennial ryegrass (Lolium perenne L.). The removal of myo-inositol from the callus culture media in combination with a cold shock pretreatment and the addition of L-Gln prior to and during Agrobacterium-infection resulted in about 84 % of the treated calluses being stably transformed. The omission of myo-inositol from the callus culture media was associated with the failure of certain pathogenesis related genes to be induced after Agrobacterium infection. The addition of a cold shock and supplemental Gln appeared to have synergistic effects on infection and transformation efficiencies. Nearly 60 % of the stably transformed calluses regenerated into green plantlets. Calluses cultured on media lacking myo-inositol also displayed profound physiological and biochemical changes compared to ones cultured on standard growth media, such as reduced lignin within the cell walls, increased starch and inositol hexaphosphate accumulation, enhanced Agrobacterium binding to the cell surface, and less H(2)O(2) production after Agrobacterium infection. Furthermore, the cold treatment greatly reduced callus browning after infection. The simple modifications described in this report may have broad application for improving genetic transformation of recalcitrant monocot species.
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