ArticleNature cell biology2013
The ontogeny of cKIT+ human primordial germ cells proves to be a resource for human germ line reprogramming, imprint erasure and in vitro differentiation.
Article in Nature cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 181 citations in OpenAlex.
- Post-translational modification of proteins in the human testis development pathway.Human reproduction update · 2026Review
- Protocol of Human Primordial Germ Cell-like Cell Generation from Pluripotent Stem Cells in 2D and 3D Culture Systems.Biology · 2026Article
- A spatiotemporal transcriptomic atlas of porcine (Sus scrofa) female early gonadal development.Communications biology · 2026Article
- Epigenetic regulation in early embryogenesis: mechanisms, developmental programming, and transgenerational implications.Frontiers in cell and developmental biology · 2026Review
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- Primordial germ cell DNA demethylation and development require DNA translesion synthesis.Nature communications · 2024Article
- Review
- Population Dynamics, Plasma Cytokines and Platelet Centrifugation: Technical and Sociodemographic Aspects of 'Ovarian Rejuvenation'.Clinics and practice · 2023Article
- Histogenesis of intracranial germ cell tumors: primordial germ cell vs. embryonic stem cell.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2023Review
- ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation.Autophagy · 2022Article
- The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors.Nature communications · 2022Article
- FGFR3 is expressed by human primordial germ cells and is repressed after meiotic initiation to form primordial oocytes.Stem cell reports · 2022Article
- Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions.Stem cell reviews and reports · 2022Article
- Transcriptional control of human gametogenesis.Human reproduction update · 2022Article
- Exposures during the prepuberty period and future offspring's health: evidence from human cohort studies†.Biology of reproduction · 2021Review
- PI3K/PTEN/AKT Signaling Pathways in Germ Cell Development and Their Involvement in Germ Cell Tumors and Ovarian Dysfunctions.International journal of molecular sciences · 2021Review
- Divergent roles for KLF4 and TFCP2L1 in naive ground state pluripotency and human primordial germ cell development.Stem cell research · 2021Article
- Germ Cell Derivation from Pluripotent Stem Cells for Understanding In Vitro Gametogenesis.Cells · 2021Review
- To Be or Not to Be a Germ Cell: The Extragonadal Germ Cell Tumor Paradigm.International journal of molecular sciences · 2021Review
45 more citing papers are in PubMed but not listed here.
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7 authors at 3 institutions in 1 country.
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Abstract
The generation of research-quality, clinically relevant cell types in vitro from human pluripotent stem cells requires a detailed understanding of the equivalent human cell types. Here we analysed 134 human embryonic and fetal samples from 6 to 20 developmental weeks and identified the stages at which cKIT(+) primordial germ cells (PGCs), the precursors of gametes, undergo whole-genome epigenetic reprogramming with global depletion of 5mC, H3K27me3 and H2A.Z, and the time at which imprint erasure is initiated and 5hmC is present. Using five alternative in vitro differentiation strategies combined with single-cell microfluidic analysis and a bona fide human cKIT(+) PGC signature, we show the stage of cKIT(+) PGC formation in the first 16 days of differentiation. Taken together, our study creates a resource of human germ line ontogeny that is essential for future studies aimed at in vitro differentiation and unveiling the mechanisms necessary to pass human DNA from one generation to the next.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.