ArticleApplied and environmental microbiology2013
Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 123 citations in OpenAlex.
- Metabolic engineering ofSynthetic and systems biotechnology · 2027Article
- Anaerobic metabolic evolution for homotypic L-valine fermentation.Nature communications · 2026Article
- Omics studies reveal the response mechanisms of3 Biotech · 2025Article
- Branched-chain amino acids: physico-chemical properties, industrial synthesis and role in signaling, metabolism and energy production.Amino acids · 2024Review
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- Reduction of acetate synthesis, enhanced arginine export, and supply of precursors, cofactors, and energy for improved synthesis of L-arginine by Escherichia coli.Applied microbiology and biotechnology · 2023Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids.Microbial cell factories · 2021Review
- L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects.Amino acids · 2021Review
- Engineering of microbial cells for L-valine production: challenges and opportunities.Microbial cell factories · 2021Review
- Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid.Microbial cell factories · 2021Article
- Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions.Applied microbiology and biotechnology · 2021Review
- TheMicroorganisms · 2021Article
- Coexistence of the Entner-Doudoroff and Embden-Meyerhof-Parnas pathways enhances glucose consumption of ethanol-producing Corynebacterium glutamicum.Biotechnology for biofuels · 2021Article
- Application of RecET-Cre/loxP system in Corynebacterium glutamicum ATCC14067 for L-leucine production.Biotechnology letters · 2021Article
- Rational modification of the carbon metabolism of Corynebacterium glutamicum to enhance L-leucine production.Journal of industrial microbiology & biotechnology · 2020Article
- Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.Metabolic engineering · 2020Article
- Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system.Microbial cell factories · 2020Article
- Improvement of l-Valine Production by Atmospheric and Room Temperature Plasma Mutagenesis and High-Throughput Screening inACS omega · 2020Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated efficient L-valine production by metabolically engineered Corynebacterium glutamicum under oxygen deprivation. To achieve the high productivity, a NADH/NADPH cofactor imbalance during the synthesis of l-valine was overcome by engineering NAD-preferring mutant acetohydroxy acid isomeroreductase (AHAIR) and using NAD-specific leucine dehydrogenase from Lysinibacillus sphaericus. Lactate as a by-product was largely eliminated by disrupting the lactate dehydrogenase gene ldhA. Nonetheless, a few other by-products, particularly succinate, were still produced and acted to suppress the L-valine yield. Eliminating these by-products therefore was deemed key to improving theL-valine yield. By additionally disrupting the phosphoenolpyruvate carboxylase gene ppc, succinate production was effectively suppressed, but both glucose consumption and L-valine production dropped considerably due to the severely elevated intracellular NADH/NAD(+) ratio. In contrast, this perturbed intracellular redox state was more than compensated for by deletion of three genes associated with NADH-producing acetate synthesis and overexpression of five glycolytic genes, including gapA, encoding NADH-inhibited glyceraldehyde-3-phosphate dehydrogenase. Inserting feedback-resistant mutant acetohydroxy acid synthase and NAD-preferring mutant AHAIR in the chromosome resulted in higher L-valine yield and productivity. Deleting the alanine transaminase gene avtA suppressed alanine production. The resultant strain produced 1,280 mM L-valine at a yield of 88% mol mol of glucose(-1) after 24 h under oxygen deprivation, a vastly improved yield over our previous best.
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