ArticleThe Journal of cell biology1990
Mechanism of the formation of contractile ring in dividing cultured animal cells. I. Recruitment of preexisting actin filaments into the cleavage furrow.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
52 citing papers in PubMed, 157 citations in OpenAlex.
- An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts.Journal of cell science · 2025Article
- Article
- Counting actin in contractile rings reveals novel contributions of cofilin and type II myosins to fission yeast cytokinesis.Molecular biology of the cell · 2022Article
- Functions of the Plant Qbc SNARE SNAP25 in Cytokinesis and Biotic and Abiotic Stress Responses.Molecules and cells · 2020Article
- Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated.BioMed research international · 2017Article
- Modest Static Pressure Suppresses Columnar Epithelial Cell Growth in Association with Cell Shape and Cytoskeletal Modifications.Frontiers in physiology · 2017Article
- Modeling the Mechanics of Cell Division: Influence of Spontaneous Membrane Curvature, Surface Tension, and Osmotic Pressure.Frontiers in physiology · 2017Article
- Article
- The value of mechanistic biophysical information for systems-level understanding of complex biological processes such as cytokinesis.Biophysical journal · 2014Review
- Capping protein regulators fine-tune actin assembly dynamics.Nature reviews. Molecular cell biology · 2014Review
- An equatorial contractile mechanism drives cell elongation but not cell division.PLoS biology · 2014Article
- The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.The Journal of cell biology · 2013Article
- ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.Nature communications · 2013Article
- Article
- Understanding cytokinesis failure.Advances in experimental medicine and biology · 2010Review
- Profilin 1 is required for abscission during late cytokinesis of chondrocytes.The EMBO journal · 2009Article
- Redistribution of actin during assembly and reassembly of the contractile ring in grasshopper spermatocytes.PloS one · 2009Article
- Nuf, a Rab11 effector, maintains cytokinetic furrow integrity by promoting local actin polymerization.The Journal of cell biology · 2008Article
- Vesicles and actin are targeted to the cleavage furrow via furrow microtubules and the central spindle.The Journal of cell biology · 2008Article
- The stress and strain of cytokinesis.Trends in cell biology · 2005Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Cytokinesis of animal cells involves the formation of the circumferential actin filament bundle (contractile ring) along the equatorial plane. To analyze the assembly mechanism of the contractile ring, we microinjected a small amount of rhodamine-labeled phalloidin (rh-pha) or rhodamine-labeled actin (rh-actin) into dividing normal rat kidney cells. rh-pha was microinjected during prometaphase or metaphase to label actin filaments that were present at that stage. As mitosis proceeded into anaphase, the labeled filaments became associated with the cortex of the cell. During cytokinesis, rh-pha was depleted from polar regions and became highly concentrated into the equatorial region. The distribution of total actin filaments, as revealed by staining the whole cell with fluorescein phalloidin, showed a much less pronounced difference between the polar and the equatorial regions. The sites of de novo assembly of actin filaments during the formation of the contractile ring were determined by microinjecting rh-actin shortly before cytokinesis, and then extracting and fixing the cell during mid-cytokinesis. Injected rhodamine actin was only slightly concentrated in the contractile ring, as compared to the distribution of total actin filaments. Our results indicate that preexisting actin filaments, probably through movement and reorganization, are used preferentially for the formation of the contractile ring. De novo assembly of filaments, on the other hand, appears to take place preferentially outside the cleavage furrow.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.