ArticleThe Journal of cell biology1990
Chemoattractant-stimulated polymorphonuclear leukocytes contain two populations of actin filaments that differ in their spatial distributions and relative stabilities.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 163 citations in OpenAlex.
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- Cytochalasin B Modulates Nanomechanical Patterning and Fate in Human Adipose-Derived Stem Cells.Cells · 2022Article
- Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking.Scientific reports · 2020Article
- Motile Human Neutrophils Sense Ligand Density Over Their Entire Contact Area.Annals of biomedical engineering · 2016Article
- Temperature oscillations drive cycles in the activity of MMP-2,9 secreted by a human trabecular meshwork cell line.Investigative ophthalmology & visual science · 2015Article
- Ligand density elicits a phenotypic switch in human neutrophils.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- State of the art in platelet function testing.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2013Article
- Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Promotion of Ccn2 expression and osteoblastic differentiation by actin polymerization, which is induced by laminar fluid flow stress.Journal of cell communication and signaling · 2012Article
- Regulation of glucose transport by ROCK1 differs from that of ROCK2 and is controlled by actin polymerization.Endocrinology · 2012Article
- Identification of a benzamide derivative that inhibits stress-induced adrenal corticosteroid synthesis.Molecules (Basel, Switzerland) · 2009Article
- p53-cofactor JMY is a multifunctional actin nucleation factor.Nature cell biology · 2009Article
- Clocking the Lyme spirochete.PloS one · 2008Article
- Perturbation of actin dynamics induces NF-kappaB activation in myelomonocytic cells through an NADPH oxidase-dependent pathway.The Biochemical journal · 2005Article
- The mechanics of neutrophils: synthetic modeling of three experiments.Biophysical journal · 2003Article
- Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis.The Journal of cell biology · 2003Article
- G protein-coupled receptor/arrestin3 modulation of the endocytic machinery.The Journal of cell biology · 2002Article
- Profilin enhances Cdc42-induced nucleation of actin polymerization.The Journal of cell biology · 2000Article
- Two pathways through Cdc42 couple the N-formyl receptor to actin nucleation in permeabilized human neutrophils.The Journal of cell biology · 2000Article
- Deactivation of neutrophil NADPH oxidase by actin-depolymerizing agents in a cell-free system.The Biochemical journal · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chemoattractants stimulate actin polymerization in lamellipodia of polymorphonuclear leukocytes. We find that removal of chemoattractant results in rapid (within 10 s at 37 degrees C) and selective depolymerization of the F-actin located in lamellipodia. Addition of 10 microM cytochalasin B, in the presence of chemoattractant, also resulted in rapid and selective depolymerization of lamellar F-actin. The elevated F-actin level induced by chemoattractant rapidly returns to the level present in unstimulated cells after (a) a 10-fold decrease in chemoattractant concentration; (b) the addition of 10 microM cytochalasin B; or (c) cooling to 4 degrees C. The F-actin levels of unstimulated cells are only slightly affected by these treatments. Based on the similar effects of cytochalasin addition and chemoattractant dilution, it is likely that both treatments result in actin depolymerization from the pointed ends of filaments. Based on our results we propose that chemoattractant-stimulated polymorphonuclear leukocytes contain two distinct populations of actin filaments. The actin filaments within the lamellipodia are highly labile and in the continued presence of chemoattractant these filaments are rapidly turning over, continually polymerizing at their plus (barbed) ends, and depolymerizing at their minus ends. In contrast, the cortical F-actin filaments of both stimulated and unstimulated cells are differentially stable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.