ArticleAmerican journal of physiology. Lung cellular and molecular physiology2013
Ozone exposed epithelial cells modify cocultured natural killer cells.
Article in American journal of physiology. Lung cellular and molecular physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of Air Pollution on Lung Innate Lymphoid Cells: Review of In Vitro and In Vivo Experimental Studies.International journal of environmental research and public health · 2019Pooled it
- Trial
- Mild Ozone-Induced Oxidative Stress Modulates the Activity and Viability of Porcine Neutrophils and Monocytes.Animals : an open access journal from MDPI · 2026Article
- Current Challenges to Align Inflammatory Key Events in Animals and Lung Cell ModelsChemical research in toxicology · 2024Review
- Parenchymal and Inflammatory Cell Responses to Single and Repeated Ozone Exposure in Healthy and Surfactant Protein-C Mutant Lung.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Oxysterols Modify NLRP2 in Epithelial Cells, Identifying a Mediator of Ozone-induced Inflammation.American journal of respiratory cell and molecular biology · 2021Article
- Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infection.Physiological reports · 2021Article
- Innovative preclinical models for pulmonary drug delivery research.Expert opinion on drug delivery · 2020Review
- Small Molecule Antipsychotic Aripiprazole Potentiates Ozone-Induced Inflammation in Airway Epithelium.Chemical research in toxicology · 2019Article
- Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Ozone-derived Oxysterols Affect Liver X Receptor (LXR) Signaling: A POTENTIAL ROLE FOR LIPID-PROTEIN ADDUCTS.The Journal of biological chemistry · 2016Article
- Cutting Edge: Role of NK Cells and Surfactant Protein D in Dendritic Cell Lymph Node Homing: Effects of Ozone Exposure.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Analysis of sphingosine kinase activity in single natural killer cells from peripheral blood.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Interaction with epithelial cells modifies airway macrophage response to ozone.American journal of respiratory cell and molecular biology · 2015Article
- Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale.BioResearch open access · 2015Article
- Article
- Culturing of human nasal epithelial cells at the air liquid interface.Journal of visualized experiments : JoVE · 2013Article
- DNA methylation in nasal epithelial cells from smokers: identification of ULBP3-related effects.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Diesel exhaust particles modify natural killer cell function and cytokine release.Particle and fibre toxicology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Ozone (O3) causes significant adverse health effects worldwide. Nasal epithelial cells (NECs) are among the first sites within the respiratory system to be exposed to inhaled air pollutants. They recruit, activate, and interact with immune cells via soluble mediators and direct cell-cell contacts. Based on our recent observation demonstrating the presence of natural killer (NK) cells in nasal lavages, the goal of this study was to establish a coculture model of NECs and NK cells and examine how exposure to O3 modifies this interaction. Flow cytometry analysis was used to assess immunophenotypes of NK cells cocultured with either air- or O3-exposed NECs. Our data show that coculturing NK cells with O3-exposed NECs decreased intracellular interferon-γ (IFN-γ), enhanced, albeit not statistically significant, IL-4, and increased CD16 expression on NK cells compared with air controls. Additionally, the cytotoxicity potential of NK cells was reduced after coculturing with O3-exposed NECs. To determine whether soluble mediators released by O3-exposed NECs caused this shift, apical and basolateral supernatants of air- and O3-exposed NECs were used to stimulate NK cells. While the conditioned media of O3-exposed NECs alone did not reduce intracellular IFN-γ, O3 enhanced the expression of NK cell ligands ULBP3 and MICA/B on NECs. Blocking ULBP3 and MICA/B reversed the effects of O3-exposed NECs on IFN-γ production in NK cells. Taken together, these data showed that interactions between NECs and NK cells in the context of O3 exposure changes NK cell activity via direct cell-cell interactions and is dependent on ULBP3/MICA/B expressed on NECs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.